Cloning and analysis of the soybean MEKK gene
| Autor: | Dezheng Qiu, Aihua Sha, Hai F. Chen, Yinhua Chen, H.-P. Ba, Xin A Zhou, Chen Shuilian, Zhihui Shan |
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| Rok vydání: | 2015 |
| Předmět: |
Signal peptide
Nitrogen Biology Gene Expression Regulation Plant Genetics Arabidopsis thaliana Cloning Molecular Molecular Biology Gene Protein secondary structure Plant Proteins Regulation of gene expression Cloning Gene Expression Profiling fungi food and beverages Phosphorus General Medicine Exons biology.organism_classification MAP Kinase Kinase Kinases Plants Genetically Modified Introns Cell biology Droughts Gene expression profiling Transmembrane domain Soybeans |
| Zdroj: | Genetics and molecular research : GMR. 14(2) |
| ISSN: | 1676-5680 |
| Popis: | In this paper, homologous cloning methods were used to clone the soybean GmMEKK gene, which possesses a high degree of similarity to Arabidopsis thaliana AtMEKK1. AtMEKK1 is formed by 595 amino acids, and its secondary structure is formed by 38 irregular curls, 24 α helix, 14 β, with S-TKc domain, transmembrane domain and does not have membrane spanning domain and signal peptide. GmMEKK-GFP subcellular localization fusion and prokaryotic expression vectors were generated and it was revealed that GmMEKK encodes a highly conserved 66.8-kDa nuclear protein that is expressed in soybean roots, stem floral pieces, and leaves. A real-time quantitative PCR analysis of GmMEKK under different abiotic stresses revealed that the expression level of GmMEKK increased under drought and low phosphorus and nitrogen conditions. Taken together, these data suggest that GmMEKK may play an important role in the soybean abiotic stress response. |
| Databáze: | OpenAIRE |
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