GPR40 is partially required for insulin secretion following activation of β3-adrenergic receptors
| Autor: | Roberta Avallone, Zhen Pang, Michael J. Tocci, Pascual Ferrara, Holly Dressler, Xin Zhang, Tiziano Croci, H. Gregory Polites, Vaseem Palejwala, Nancy N. Wu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Agonist endocrine system medicine.medical_specialty Tetrahydronaphthalenes Adrenergic receptor medicine.drug_class medicine.medical_treatment Adrenergic beta-3 Receptor Agonists Stimulation Dioxoles Biology Biochemistry Receptors G-Protein-Coupled Eating Mice Endocrinology Free fatty acid receptor 1 Internal medicine Insulin Secretion medicine Animals Hypoglycemic Agents Insulin Lipolysis Receptor Molecular Biology Mice Knockout Dose-Response Relationship Drug Body Weight Adrenergic beta-Agonists C++ AMP Mice Inbred C57BL Receptors Adrenergic beta-3 Energy Metabolism |
| Zdroj: | Molecular and Cellular Endocrinology. 325:18-25 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2010.04.014 |
| Popis: | The free fatty acid (FFA) receptor GPR40, expressed by pancreatic beta-cells, may be responsible for insulin release following beta(3) adrenoceptor (Adrb3) activation. To test this hypothesis, we first studied the effects of Adrb3 agonists SR58611A and CL316,243 in GPR40 knockout (GPR40(-/-)) mice. Both drugs increased blood FFA levels in wild-type (GPR40(+/+)) and GPR40(-/-) mice, indicating that lipolysis is not GPR40-dependent. However, the magnitude of the insulin response after agonist treatment was decreased by approximately 50% in GPR40(-/-) mice. Analysis of the time-course revealed that the change in FFAs (5-10 min post-treatment) in response to SR58611A preceded insulin secretion (10-15 min post-treatment). While reduced by agonist treatment, glucose levels in GPR40(-/-) mice remained significantly higher than in GPR40(+/+) mice. Energy expenditure, food intake, or body weight was not affected in GPR40(-/-) mice, whereas SR58611A increased energy metabolism. Furthermore, CL316,243 did not potentiate glucose-stimulated insulin secretion in isolated mouse islets or activate a cAMP reporter in transgenic mice. Our data indicate that insulin secretion, a secondary event following stimulation of Adrb3 receptors, is partially mediated by GPR40 and suggest that GPR40 is integral to the anti-diabetes effects of Adrb3 agonists. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect