Endothelial nitric oxide synthase (ecNOS) 4 a/b gene polymorphism and carotid artery intima-media thickness in type-1 diabetic patients
| Autor: | M. Pfohl, Chitu J, Frost D, Meyer M, Wolfgang Beischer |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Genotype Nitric Oxide Synthase Type III Arteriosclerosis Endocrinology Diabetes and Metabolism Nephropathy Endocrinology Gene Frequency Internal medicine Internal Medicine medicine Humans Alleles Ultrasonography Diabetic Retinopathy Polymorphism Genetic business.industry Reverse Transcriptase Polymerase Chain Reaction General Medicine Odds ratio Diabetic retinopathy DNA medicine.disease Carotid Arteries Diabetes Mellitus Type 1 Intima-media thickness Albuminuria Electrophoresis Polyacrylamide Gel Female Gene polymorphism medicine.symptom Nitric Oxide Synthase business Retinopathy |
| Zdroj: | Experimental and clinical endocrinologydiabetes : official journal, German Society of Endocrinology [and] German Diabetes Association. 111(1) |
| ISSN: | 0947-7349 |
| Popis: | AIM To study putative associations of the ecNOS 4 a/b polymorphism with carotid artery intima-media thickness (IMT) and diabetic complications in young type-1 diabetic patients. METHODS Study participants were 147 type-1 diabetic patients (56 men and 91 women), mean age 30.1 +/- 6.6 years (range 14 - 44), with a diabetes duration of 13.1 +/- 8.1 years. HbA1c, albuminuria, and lipid status were assessed by standard laboratory techniques, the ecNOS 4 a/b genotype was determined by polymerase chain reaction with subsequent polyacrylamide gel electrophoresis. The patients were categorized according to the presence or absence of hypertension, nephropathy and retinopathy. The IMT, which can be used to estimate early stages of arteriosclerosis, was measured by high-resolution ultrasonography. RESULTS The ecNOS genotypes were distributed as follows: 7.5 % a/a, 30.6 % a/b, and 61.9 % b/b. The IMT values did not differ between the patients with various ecNOS genotypes (a/a: 0.62 +/- 0.13; a/b: 0.63 +/- 0.21; b/b: 0.63 +/- 0.13; all: 0.63 +/- 0.15 mm). The prevalence of retinopathy was significantly higher in patients with the b/b genotype (odds ratio: 2.4 vs. a/a+a/b; 95 % CI, 1.1 - 5.3). CONCLUSIONS Our results do not support the hypothesis that the ecNOS 4 a/b polymorphism interacts with the development of early carotid arteriosclerosis in young type-1 diabetic patients, but they give grounds to assume that in these patients it could influence the occurence of diabetic retinopathy. |
| Databáze: | OpenAIRE |
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