Aberrant immunoglobulin G glycosylation in multiple sclerosis
| Autor: | Stefan Sillau, Xiaomeng Li, Tiffany Pointon, Wenbo Zhou, Anthony Fringuello, Michael W. Graner, Kathryn Dennison, Gina Im, Kayo Tanimoto, Xiaoli Yu, Arin N. Graner, Timothy Vollmer, Peter G. E. Kennedy |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Glycosylation Immunology Neuroscience (miscellaneous) Inflammation Immunoglobulin G Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cerebrospinal fluid medicine Immunology and Allergy Pharmacology biology Chemistry Multiple sclerosis Lectin Biological activity medicine.disease carbohydrates (lipids) 030104 developmental biology biology.protein medicine.symptom Antibody 030217 neurology & neurosurgery |
| Zdroj: | J Neuroimmune Pharmacol |
| ISSN: | 1557-1890 |
| Popis: | A hallmark of the inflammatory response in multiple sclerosis (MS) is the presence of intrathecal Immunoglobulin G (IgG) antibodies and oligoclonal bands (OCBs). The biological activity of IgGs is modulated by changes in glycosylation. Using multiple immunoassays with common lectins for sialylation and galactosylation, we investigated levels of IgG glycosylation in 28 MS and 37 control sera as well as paired CSF and serum. We demonstrated the presence of significantly lower levels of IgG sialylation in MS CSF compared to paired serum. Further, we showed that in MS there was no correlation between sialylated IgG and total IgG antibodies, or between sialylated IgG in CSF and serum. ELISA with native IgG antibodies showed significantly higher levels of sialylated and galactosylated IgG in MS compared to other neurological disorders and normal healthy controls. We conclude that lower levels of sialylated intrathecal IgG and higher levels of serum IgG galactosylation in MS may play significant role in disease pathogenesis. The unique IgG glycosylation profiles suggest a complexed nature of the IgG antibodies which may influence its effector functions in MS. |
| Databáze: | OpenAIRE |
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