Seladin-1/DHCR24 expression in normal ovary, ovarian epithelial and granulosa tumours
| Autor: | Tom Jobling, Peter J. Fuller, Maria Alexiadis, Jane McNeilage |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Adult
Oxidoreductases Acting on CH-CH Group Donors medicine.medical_specialty Pathology Adolescent endocrine system diseases Serous cystadenocarcinoma Endocrinology Diabetes and Metabolism Granulosa cell Nerve Tissue Proteins Ovary Biology Cystadenocarcinoma Mucinous Ovarian tumor Endocrinology Internal medicine medicine Humans Ovarian follicle Aged Granulosa Cell Tumor Aged 80 and over Ovarian Neoplasms Reverse Transcriptase Polymerase Chain Reaction Middle Aged Receptors LH medicine.disease Epithelium Cystadenocarcinoma Serous Neoplasm Proteins Gene Expression Regulation Neoplastic Blotting Southern medicine.anatomical_structure Female Mucinous cystadenocarcinoma Hormone |
| Zdroj: | Clinical Endocrinology. 63:111-115 |
| ISSN: | 1365-2265 0300-0664 |
| DOI: | 10.1111/j.1365-2265.2005.02308.x |
| Popis: | Summary Objective The human DIMINUTO/DWARF1 homolog seladin-1/DHCR24 has been recently reported to be up-regulated in adrenocortical adenomas. Seladin-1 expression has been reported in the normal ovary. Granulosa cell tumours of the ovary (GCT) as with adrenocortical adenomas arise from a steroidogenic tissue, respond to pituitary hormone stimulation and synthesize steroid hormones. Design To test the hypothesis that seladin-1 may also have a role in the pathogenesis of GCT, we determined the expression of seladin-1 in a cohort of GCT and in mucinous and serous cystadenocarcinomas and in normal ovary. Measurements Expression was determined by RT-PCR using gene specific primers and probes combined with Southern blot analysis of the PCR products. Results Seladin-1 expression was identified in the normal ovary, mucinous cystadenocarcinomas and serous cystadenocarcinomas of the ovary whereas no expression was observed in the GCT. Conclusions Based on our results, seladin-1 is not expressed in the granulosa cells or at least not in those that give rise to GCT. |
| Databáze: | OpenAIRE |
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