Chiral separation by high performance liquid chromatography. I. Review on indirect separation of enantiomers as diastereomeric derivatives using ultraviolet, fluorescence and electrochemical detection
| Autor: | Linus N. Igwemezie, Nuggehally R. Srinivas |
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| Rok vydání: | 1992 |
| Předmět: |
Pharmacology
Chromatography Chemistry Clinical Biochemistry Diastereomer Stereoisomerism General Medicine Electrochemical detection Biochemistry High-performance liquid chromatography Fluorescence Analytical Chemistry Spectrometry Fluorescence Reagent Drug Discovery Electrochemistry Spectrophotometry Ultraviolet Enantiomer Ultraviolet fluorescence Molecular Biology Chromatography High Pressure Liquid |
| Zdroj: | Biomedical chromatography : BMC. 6(4) |
| ISSN: | 0269-3879 |
| Popis: | The increased attention on the therapeutic implications of stereoisomerism has provided an impetus for the development of analytical methods for enantiomeric separation. The indirect method of separation of enantiomers as diastereomers using high performance liquid chromatography (HPLC) has emerged as an efficient and versatile approach. This is due mainly to the availability of numerous chiral derivatization reagents (CDRs). This article reviews CDRs useful for the development of an indirect HPLC method using ultraviolet, fluorescence and electrochemical detection. In addition, factors crucial for the development of the indirect method are discussed. |
| Databáze: | OpenAIRE |
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