DDX10 overexpression predicts worse prognosis in osteosarcoma and its deletion prohibits cell activities modulated by MAPK pathway
| Autor: | Jian-Hui Shi, Ying-Jie Hao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway MAP Kinase Signaling System Cell Biophysics Bone Neoplasms Biology Biochemistry DEAD-box RNA Helicases 03 medical and health sciences 0302 clinical medicine Gentamicin protection assay Cell Line Tumor medicine Gene silencing Humans Neoplasm Invasiveness Viability assay Molecular Biology Cell Proliferation Osteosarcoma Migration Assay Cell migration Cell Biology medicine.disease Prognosis Up-Regulation 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research Gene Deletion |
| Zdroj: | Biochemical and biophysical research communications. 510(4) |
| ISSN: | 1090-2104 |
| Popis: | Osteosarcoma (OS) is an invasive cancer in the skeletal system. The molecular mechanism of its etiology and pathogenesis are still not clear, so the effective treatment strategy of OS needs further research. First, we analyzed the expression level and prognostic ability of the RNA helicase DDX10 in OS patients based on the data obtained from GEO database. Next, we used CCK8 to test OS cell viability. Besides, we used wound-healing assay and transwell migration assay to detect cell migration of OS MG63 cell line. And the cell invasion was tested by transwell invasion assay. Moreover, we used QRT-PCR and western blot to analyze the mRNA and protein expression levels. We found that DDX10 was significantly over-expressed in OS patients and elevated level of DDX10 was associated with a poor prognosis. Silencing of DDX10 inhibited proliferation, invasion and migration of MG63 cells in vitro. Down-regulation of DDX10 inhibited MAPK signaling pathway. The expression of p-MEK and p-ERK were also decreased by silencing of DDX10. Therefore, Silencing of DDX10 inhibited proliferation, invasion and migration of MG63 cells, which might be regulated by suppression of MAPK pathway. In conclusion, our results unfold a novel area of studying for understanding how DDX10 functions in OS oncogenic and prognostic significance, accordingly implying a promising therapeutic target for OS treatment. |
| Databáze: | OpenAIRE |
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