DNA methylation as mechanism of apoptotic resistance development in endometrial cancer patients
| Autor: | Jozef Hatok, Juraj Marcinek, Sandra Hanysova, Dusan Dobrota, Tomas Balharek, Erik Kudela, Veronika Fialkova, Jozef Visnovsky, Eva Vidomanová |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oncology Adult medicine.medical_specialty Slovakia Physiology Carcinogenesis Biophysics Apoptosis Biology medicine.disease_cause Polymorphism Single Nucleotide Epigenesis Genetic 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Humans Genetic Predisposition to Disease Epigenetics Gene Aged Regulation of gene expression Endometrial cancer Promoter General Medicine Methylation DNA Neoplasm DNA Methylation Middle Aged medicine.disease Endometrial Neoplasms 030104 developmental biology 030220 oncology & carcinogenesis DNA methylation Cancer research Female Apoptosis Regulatory Proteins Precancerous Conditions |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0231-5882 |
| Popis: | DNA methylation is a significant epigenetic modification which plays a key role in regulation of gene expression and influences functional changes in endometrial tissue. Aberrant DNA methylation changes result in deregulation of important apoptotic proteins during endometrial carcinogenesis and apoptosis resistance development. Evading apoptosis is still a major problem in the successful treatment of endometrial cancer patients. The aim of our study was to examine the promoter DNA methylation changes in 22 apoptosis-associated genes in endometrioid endometrial cancer patients, precancerous lesions and healthy tissue from various normal menstrual cycle phases using a unique pre-designed methylation platform. We observed as the first a significant difference in promoter DNA methylation status in genes: BCL2L11 (p < 0.001), CIDEB (p < 0.03) and GADD45A (p < 0.05) during endometrial carcinogenesis and BIK gene (p < 0.03) in different phases of normal menstrual cycle. The results of our study indicate that deregulation of mitochondrial apoptotic pathway can considerably contributes to the apoptosis resistance development and may be helpful in identifying of new potent biomarkers in endometrial cancer. |
| Databáze: | OpenAIRE |
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