Glycogen in Bacillus subtilis. Molecular characterization of an operon encoding enzymes involved in glycogen biosynthesis and degradation
| Autor: | J. A. K. W. Kiel, J. M. Boels, G. Beldman, Gerhardus Venema |
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| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
DNA
Bacterial Phosphorylases Operon Molecular Sequence Data Restriction Mapping Glucose-1-Phosphate Adenylyltransferase Bacillus subtilis Microbiology Open Reading Frames chemistry.chemical_compound 1 4-alpha-Glucan Branching Enzyme Food Chemistry and Microbiology Glycogen branching enzyme gal operon Life Science Amino Acid Sequence Cloning Molecular Molecular Biology Spores Bacterial Base Sequence biology Glycogen Gene Expression Regulation Bacterial Sequence Analysis DNA Metabolism Chromosomes Bacterial biology.organism_classification Nucleotidyltransferases Glycogen Synthase Levensmiddelenchemie en -microbiologie Biochemistry chemistry Genes Bacterial biology.protein bacteria L-arabinose operon Sequence Alignment Bacteria |
| Zdroj: | Molecular Microbiology 11 (1994) Molecular Microbiology, 11, 203-218 |
| ISSN: | 0950-382X |
| Popis: | Although it has never been reported that Bacillus subtilis is capable of accumulating glycogen, we have isolated a region from the chromosome of B. subtilis containing a glycogen operon. The operon is located directly downstream from trnB, which maps at 275 degrees on the B. subtilis chromosome. It encodes five polypeptides with extensive similarity to enzymes involved in glycogen and starch metabolism in both prokaryotes and eukaryotes. The operon is presumably expressed by an E sigma(E)-controlled promoter, which was previously identified downstream from trnB. We have observed glycogen biosynthesis in B. subtilis exclusively on media containing carbon sources that allow efficient sporulation. Sporulation-independent synthesis of glycogen occurred after integration of an E sigma(A) controlled promoter upstream of the operon. |
| Databáze: | OpenAIRE |
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