Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF
| Autor: | Kavindra V. Singh, Cesar A. Arias, Agathe Bourgogne, Donna M. Muzny, Shahreen Chowdhury, Huaiyang Jiang, Jason Gioia, Vittal P. Prakash, Kristina A. Fox, Lei Chen, Yan Ding, Barbara E. Murray, Richard A. Gibbs, Gabrielle Williams, Yue Shang, Sreedhar R. Nallapareddy, Hua Shen, Danielle A. Garsin, Sarah K. Highlander, Xiang Qin, Arash Maadani, George M. Weinstock, Shailaja Yerrapragada, Shannon Dugan-Rocha, Jouko Sillanpää, Meng Zhao, Guan Chen, Christian J. Buhay |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
DNA
Bacterial Virulence Genomics Genome Enterococcus faecalis Microbiology Mice 03 medical and health sciences Bacterial Proteins Sequence Homology Nucleic Acid Drug Resistance Bacterial Operon Genetic variation Animals Pathogen Repetitive Sequences Nucleic Acid 030304 developmental biology Genetics 0303 health sciences biology 030306 microbiology Research Strain (biology) Genetic Variation Membrane Proteins biology.organism_classification Anti-Bacterial Agents 3. Good health Interspersed Repetitive Sequences Biofilms Scale variation Rifampin Fusidic Acid human activities Genome Bacterial |
| Zdroj: | Genome Biology |
| ISSN: | 1465-6906 |
| DOI: | 10.1186/gb-2008-9-7-r110 |
| Popis: | A comparison of two strains of the hospital pathogen Enterococcus faecalis suggests that mediators of virulence differ between strains and that virulence does not depend on mobile gene elements Background Enterococcus faecalis has emerged as a major hospital pathogen. To explore its diversity, we sequenced E. faecalis strain OG1RF, which is commonly used for molecular manipulation and virulence studies. Results The 2,739,625 base pair chromosome of OG1RF was found to contain approximately 232 kilobases unique to this strain compared to V583, the only publicly available sequenced strain. Almost no mobile genetic elements were found in OG1RF. The 64 areas of divergence were classified into three categories. First, OG1RF carries 39 unique regions, including 2 CRISPR loci and a new WxL locus. Second, we found nine replacements where a sequence specific to V583 was substituted by a sequence specific to OG1RF. For example, the iol operon of OG1RF replaces a possible prophage and the vanB transposon in V583. Finally, we found 16 regions that were present in V583 but missing from OG1RF, including the proposed pathogenicity island, several probable prophages, and the cpsCDEFGHIJK capsular polysaccharide operon. OG1RF was more rapidly but less frequently lethal than V583 in the mouse peritonitis model and considerably outcompeted V583 in a murine model of urinary tract infections. Conclusion E. faecalis OG1RF carries a number of unique loci compared to V583, but the almost complete lack of mobile genetic elements demonstrates that this is not a defining feature of the species. Additionally, OG1RF's effects in experimental models suggest that mediators of virulence may be diverse between different E. faecalis strains and that virulence is not dependent on the presence of mobile genetic elements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|