Pattern of cutaneous immunoglobulin G deposition in subacute cutaneous lupus erythematosus is reproduced by infusing purified anti-Ro (SSA) autoantibodies into human skin-grafted mice
| Autor: | J B Harley, L.A. Lee, Silvija Coulter, K K Gaither, David A. Norris |
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| Rok vydání: | 1989 |
| Předmět: |
Adult
Male musculoskeletal diseases Transplantation Heterologous Fluorescent Antibody Technique Mice Nude Biology Autoantigens Immunoglobulin G Subacute cutaneous lupus erythematosus Mice stomatognathic system Antigen RNA Small Cytoplasmic Lupus Erythematosus Cutaneous medicine Animals Humans Autoantibodies Skin Mice Inbred BALB C Systemic lupus erythematosus Lupus erythematosus integumentary system Immunologic Deficiency Syndromes Autoantibody Infant Skin Transplantation General Medicine medicine.disease eye diseases Transplantation stomatognathic diseases Ribonucleoproteins Immunology biology.protein Female Binding Sites Antibody Epidermis Research Article Anti-SSA/Ro autoantibodies |
| Zdroj: | Journal of Clinical Investigation. 83:1556-1562 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci114052 |
| Popis: | Subacute cutaneous lupus and neonatal lupus are closely associated with the presence of anti-Ro (SSA) autoantibodies, but there is no direct evidence establishing a role for anti-Ro (SSA) in these diseases. After parental injection into mice, IgG from sera containing anti-Ro (SSA) will bind human skin grafted onto the mice. To determine whether the antibody binding is due to anti-Ro (SSA), affinity-purified anti-Ro (SSA) and serum depleted of anti-Ro (SSA) were prepared. After injection into human skin-grafted mice, purified anti-Ro (SSA) antibodies bound an antigen in the human skin graft, while preabsorbing anti-Ro (SSA) serum with Ro (SSA) virtually abolished binding to the human skin graft. Moreover, the pattern of IgG deposition was primarily epidermal and was identical in the human skin-grafted mice injected with purified anti-Ro (SSA) when compared with that found in five patients with subacute lupus (four adults, one neonate). These data directly show that anti-Ro (SSA) antibodies bind to the skin, and support the hypothesis that anti-Ro (SSA) autoantibodies are involved in the disease process that produces subacute cutaneous lupus and neonatal lupus. |
| Databáze: | OpenAIRE |
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