CRISPR/Cas9-mediated knockout of the PDEF gene inhibits migration and invasion of human gastric cancer AGS cells
| Autor: | Yun-Fei Li, Wen-Qing Hu, Xiao-su Guo, Hui-Peng Zhang, Yi-Qiang Zhang, Guo-yan Cui, Jin-Hong Pei, Shuai-Shuai Shi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Carcinogenesis RM1-950 Biology medicine.disease_cause Flow cytometry 03 medical and health sciences Random Allocation 0302 clinical medicine Invasion Cell Movement Stomach Neoplasms Cell Line Tumor medicine Humans Neoplasm Invasiveness Transcription factor CRISPR/Cas9 Cell proliferation Migration Pharmacology medicine.diagnostic_test Base Sequence Proto-Oncogene Proteins c-ets Cell growth Cancer PDEF General Medicine medicine.disease Epithelium 030104 developmental biology medicine.anatomical_structure Apoptosis Gastric Mucosa 030220 oncology & carcinogenesis Gene Knockdown Techniques Cancer research Immunohistochemistry Therapeutics. Pharmacology CRISPR-Cas Systems Gastric cancer |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 111, Iss, Pp 76-85 (2019) |
| ISSN: | 1950-6007 |
| Popis: | Gastric cancer is one of the most common malignant tumors worldwide and has the second highest incidence and mortality rate among malignant tumors in China. Prostate-derived Ets factor (PDEF) is a member of the Ets family of transcription factors. Although PDEF plays an important role in tumorigenesis, its biological function in gastric cancer is still unclear. Here, we evaluated PDEF expression in 30 cases of human gastric carcinoma and the corresponding peritumoral tissues, using immunohistochemistry and immunofluorescence. Significantly higher levels of PDEF were detected in tumors compared to peritumoral tissues. We then investigated PDEF expression in the gastric cancer cell lines SGC and AGS and the normal gastric epithelial cell line GES; The CRISPR/Cas9 genome-editing system was used to knockout PDEF in AGS cells as a model for gastric cancer. Cell proliferation, apoptosis, migration, and invasion of PDEF-knockout AGS cells were evaluated using CCK-8, flow cytometry, scratch wound, and transwell assays, respectively. The results illustrated that PDEF-knockout inhibited AGS cell proliferation, migration, and invasion. Taken together, the results imply that PDEF plays important roles in the proliferation, migration, and invasion of AGS cells and may serve as a new treatment target in gastric cancer. |
| Databáze: | OpenAIRE |
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