Circulating microRNAs are associated with early childhood obesity: results of the I.Family Study

Autor: Ronja Foraita, Alfonso Siani, Pasquale De Luca, Fabio Lauria, Dénes Molnár, Giuseppe Iacomino, Toomas Veidebaum, Wolfgang Ahrens, Pasquale Marena, Michael Tornaritis, Kathrin Günther, Antonella Venezia, Luis A. Moreno, Lauren Lissner, Paola Russo, Stefaan De Henauw
Rok vydání: 2018
Předmět:
0301 basic medicine
Oncology
obesity
medicine.medical_specialty
lcsh:QH426-470
PLASMA MICRORNA
Endocrinology
Diabetes and Metabolism
Metabolic disorders
lcsh:TX341-641
030209 endocrinology & metabolism
Disease
Clinical nutrition
Overweight
Childhood obesity
Childhood overweight
low-grade
03 medical and health sciences
0302 clinical medicine
Diabetes mellitus
Internal medicine
Medicine and Health Sciences
Genetics
QUALITY
Medicine
2. Zero hunger
business.industry
Research
SIGNATURE
Biology and Life Sciences
EXPRESSION PROFILE
Biomarker
medicine.disease
Obesity
Childhood overweight/low-grade obesity
Circulating miRNAs
3. Good health
BODY-MASS INDEX
lcsh:Genetics
Circulating MicroRNA
030104 developmental biology
MIRNAS
medicine.symptom
business
lcsh:Nutrition. Foods and food supply
Dyslipidemia
Zdroj: Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname
Genes & Nutrition, Vol 14, Iss 1, Pp 1-13 (2019)
Genes & nutrition, 14: 2
GENES AND NUTRITION
Genes & Nutrition
Genes & nutrition 14 (2019): 1–13. doi:10.1186/s12263-018-0622-6
info:cnr-pdr/source/autori:Giuseppe Iacomino1,*, Paola Russo1, Pasquale Marena1, Fabio Lauria1, Antonella Venezia1, Wolfgang Ahrens2, Stefaan De Henauw3, Pasquale De Luca4, Ronja Foraita2, Kathrin Günther2, Lauren Lissner5, Dénes Molnár6, Luis A Moreno7, Michael Tornaritis8, Toomas Veidebaum9, and Alfonso Siani1./titolo:Circulating microRNAs are associated with early childhood obesity: results of the I.Family Study./doi:10.1186%2Fs12263-018-0622-6/rivista:Genes & nutrition/anno:2019/pagina_da:1/pagina_a:13/intervallo_pagine:1–13/volume:14
ISSN: 1555-8932
1865-3499
DOI: 10.1186/s12263-018-0622-6
Popis: Background: Nearly 10 years ago, the World Health Organization reported the increasing prevalence of overweight and obesity worldwide as a challenge for public health due to the associated adverse consequences. Epidemiological studies established a firm relationship between an elevated body mass index and chronic conditions such as diabetes, dyslipidemia, hypertension, heart disease, non-alcoholic fatty liver disease, and some types of cancer. Omic studies demonstrated that microRNA (miRNA) profile changes in tissues correlate with a number of diseases, including obesity. Recent studies showed a remarkable stability of miRNAs also in blood, emphasizing their potential as theranostic agents for a variety of disorders and conditions. A number of miRNAs enriched in homeostasis of obesity and metabolic disorders have been characterized in previous researches.Aim: This work was finalized to investigate the differential circulating miRNAs signature in early childhood obesity. Our cross-sectional study analyzed the signature of circulating miRNAs in plasma samples of normal weight (n = 159) and overweight/obese (n = 149) children and adolescents participating to the I.Family study, an EC-funded study finalized to investigate the etiology of overweight, obesity and related disorders and the determinants of food choice, lifestyle, and related health outcomes in children and adolescents of eight European countries (www.ifamilystudy.eu).Results: Differences in miRNA signature with respect to anthropometric and biochemical variables were analyzed. A high degree of variability in levels of circulating miRNAs was identified among children from different countries, in line with recent reports supporting the hypothesis that these molecules are likewise affected by environmental and lifestyle factors. A panel of miRNAs differentially expressed in overweight/low-grade obesity children was characterized (miR-551a and miR-501-5p resulted upregulated; miR-10b-5p, miR-191-3p, miR-215-5p, and miR-874-3p resulted downregulated). ROC curves were also constructed for experimentally confirmed miRNAs. Single miRNAs generally exhibited low AUC values with the highest values for miR-874-3p and miR-501-5p which in combination provided an interesting value (AUC = 0.782). Pearson's analysis confirmed that miR-10b-5p, miR-215-5p, miR-501-5p, miR-551a, and miR-874-3p significantly correlated with BMI z-score. Molecular interactions of obesity-associated miRNAs were also predicted by bioinformatics tools.Conclusions: Our work showed that several circulating miRNAs are differentially represented in overweight/low-grade obesity children and adolescents. Although causal pathways cannot be firmly inferred, it is conceivable that circulating miRNAs may be new biomarkers of early childhood obesity.Trial registration: ISRCTN, ISRCTN62310987. Registered 23/02/2018 - Retrospectively registered.
Databáze: OpenAIRE
Externí odkaz: