Improving the recovery of clarification process of recombinant hepatitis B surface antigen in large-scale by optimizing adsorption-desorption parameters on Aerosil-380
| Autor: | Seyed Nezamedin Hosseini, Parisa Ghaisari, Shahram Sharifnia, Amin Javidanbardan, Maryam Khatami |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Adsorption desorption Enzyme-Linked Immunosorbent Assay Buffers Hepatitis b surface antigen 01 natural sciences Biochemistry law.invention 03 medical and health sciences Adsorption Microscopy Electron Transmission Antigen law 010608 biotechnology Spectroscopy Fourier Transform Infrared Fumed silica Hepatitis B Surface Antigens Chromatography Kinetic model Chemistry Temperature General Medicine Hydrogen-Ion Concentration Silicon Dioxide Recombinant Proteins Kinetics 030104 developmental biology Models Chemical Scientific method Microscopy Electron Scanning Recombinant DNA Thermodynamics Electrophoresis Polyacrylamide Gel Biotechnology |
| Zdroj: | Preparative Biochemistry & Biotechnology. 48:490-497 |
| ISSN: | 1532-2297 1082-6068 |
| DOI: | 10.1080/10826068.2018.1466153 |
| Popis: | In the downstream process of recombinant hepatitis B surface antigen (rHBsAg), nano-colloidal silica adsorbent (Aerosil-380) is one of the possible methods to separate the antigen from other main impurities partially. The current study aimed to maximize the adsorptive capacity of Aerosil-380 as well as rHBsAg recovery for large-scale production of recombinant hepatitis B vaccine. The experimental design methodology was used to optimize the eight critical parameters influencing the efficiency, rHBsAg recovery, of the adsorption-desorption process in the lab-scale. These examined parameters were the adsorption-desorption temperature, pH, contact time, agitation speed, antigen concentration, and desorption buffer. Under optimal condition, the maximum adsorption capacity of Aerosil-380 was equal to 3333 μg.g |
| Databáze: | OpenAIRE |
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