Facilities for macromolecular crystallography at the Helmholtz-Zentrum Berlin
| Autor: | Karthik S. Paithankar, Uwe Mueller, Nora Darowski, Michael Steffien, Manfred S. Weiss, Georg Zocher, Michael Hellmig, Martin Fuchs, Ronald Förster, Sandra Pühringer |
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| Rok vydání: | 2011 |
| Předmět: |
Nuclear and High Energy Physics
Research groups Computer science Hydrolases Macromolecular Substances Mineralogy crystal dehydration Crystallography X-Ray law.invention high-throughput methods Mice UV radiation-damage-induced phasing law Animals long-wavelength phasing macromolecular crystallography beamlines high throughput methods UV radiation damage induced phasing long wavelength phasing Instrumentation Automation Laboratory Radiation Test facility Opsins business.industry Macromolecular crystallography Electrical engineering Beamlines Genomics Orosomucoid Synchrotron Insertion device Electron storage Ccd detector business Synchrotrons |
| Zdroj: | Journal of Synchrotron Radiation |
| ISSN: | 1600-5775 |
| Popis: | The three macromolecular crystallography beamlines BL14.1, BL14.2 and BL14.3 at the BESSY II storage ring at the Helmholtz-Zentrum Berlin are described. Three macromolecular crystallography (MX) beamlines at the Helmholtz-Zentrum Berlin (HZB) are available for the regional, national and international structural biology user community. The state-of-the-art synchrotron beamlines for MX BL14.1, BL14.2 and BL14.3 are located within the low-β section of the BESSY II electron storage ring. All beamlines are fed from a superconducting 7 T wavelength-shifter insertion device. BL14.1 and BL14.2 are energy tunable in the range 5–16 keV, while BL14.3 is a fixed-energy side station operated at 13.8 keV. All three beamlines are equipped with CCD detectors. BL14.1 and BL14.2 are in regular user operation providing about 200 beam days per year and about 600 user shifts to approximately 50 research groups across Europe. BL14.3 has initially been used as a test facility and was brought into regular user mode operation during the year 2010. BL14.1 has recently been upgraded with a microdiffractometer including a mini-κ goniometer and an automated sample changer. Additional user facilities include office space adjacent to the beamlines, a sample preparation laboratory, a biology laboratory (safety level 1) and high-end computing resources. In this article the instrumentation of the beamlines is described, and a summary of the experimental possibilities of the beamlines and the provided ancillary equipment for the user community is given. |
| Databáze: | OpenAIRE |
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