Identification of genes regulated during osteoblastic differentiation by genome-wide expression analysis of mouse calvaria primary osteoblasts in vitro
| Autor: | Katherine M. Call, Steven Bushnell, Amanda L. Jackson, S Spinella-Jaegle, Roland Baron, Georges Rawadi, Sergio Roman-Roman, Joachim Theilhaber, Marielle Auberval, Teresa Garcia, Timothy Connolly, B Courtois, Shinji Kawai |
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| Rok vydání: | 2003 |
| Předmět: |
musculoskeletal diseases
Histology Physiology Endocrinology Diabetes and Metabolism Cellular differentiation Calvaria Mice Inbred Strains Receptors Cell Surface Biology Extracellular matrix Mice Gene expression Endopeptidases medicine Animals Growth Substances Transcription factor Gene Cells Cultured Oligonucleotide Array Sequence Analysis Extracellular Matrix Proteins Genome Osteoblasts Skull Osteoblast Cell Differentiation Tissue Inhibitor of Metalloproteinases Molecular biology Cytoskeletal Proteins medicine.anatomical_structure Cell culture Cell Adhesion Molecules Transcription Factors |
| Zdroj: | Bone. 32(5) |
| ISSN: | 8756-3282 |
| Popis: | Although several independent studies of gene expression patterns during osteoblast differentiation in cultures from calvaria and other in vitro models have been reported, only a small portion of the mRNAs expressed in osteoblasts have been characterized. We have previously analyzed the behavior of several known markers in osteoblasts, using Affymetrix GeneChip murine probe arrays (27,000 genes). In the present study we report larger groups of transcripts displaying significant expression modulation during the culture of osteoblasts isolated from mice calvaria. The expression profiles of 601 such regulated genes, classified in distinct functional families, are presented and analyzed here. Although some of these genes have previously been shown to play important roles in bone biology, the large majority of them have never been demonstrated to be regulated during osteoblast differentiation. Despite the fact that the precise involvement of these genes in osteoblast differentiation and function needs to be evaluated, the data presented herein will aid in the identification of genes that play a significant role in osteoblasts. This will provide a better understanding of the regulation of osteoblast differentiation and maturation. |
| Databáze: | OpenAIRE |
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