Understanding the structural features of high-amylose maize starch through hydrothermal treatment
| Autor: | Fengwei Xie, Xiaoqin Shang, Ling Chen, Peng Liu, Wenqiang Wen, Jianing Yang |
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| Rok vydání: | 2016 |
| Předmět: |
TP
Materials science Scanning electron microscope Starch 02 engineering and technology Zea mays Biochemistry chemistry.chemical_compound Crystallinity 0404 agricultural biotechnology Differential scanning calorimetry X-Ray Diffraction Structural Biology Amylose Lamellar structure SB Molecular Biology Calorimetry Differential Scanning Small-angle X-ray scattering Hydrolysis Temperature 04 agricultural and veterinary sciences General Medicine 021001 nanoscience & nanotechnology 040401 food science Crystallography chemistry Amylopectin Thermodynamics sense organs 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 84:268-274 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2015.12.033 |
| Popis: | In this study, high-amylose starches were hydrothermally-treated and the structural changes were monitored with time (up to 12 h) using scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), small-angle X-ray scattering (SAXS), X-ray diffraction (XRD), and differential scanning calorimetry (DSC). When high-amylose starches were treated in boiling water, half-shell-like granules were observed by SEM, which could be due to the first hydrolysis of the granule inner region (CLSM). This initial hydrolysis could also immediately (0.5 h) disrupt the semi-crystalline lamellar regularity (SAXS) and dramatically reduce the crystallinity (XRD); but with prolonged time of hydrothermal treatment (≥2 h), might allow the perfection or formation of amylose single helices, resulting in slightly increased crystallinity (XRD and DSC). These results show that the inner region of granules is composed of mainly loosely-packed amylopectin growth rings with semi-crystalline lamellae, which are vulnerable under gelatinization or hydrolysis. In contrast, the periphery is demonstrated to be more compact, possibly composed of amylose and amylopectin helices intertwined with amylose molecules, which require greater energy input (higher temperature) for disintegration.\ud \ud |
| Databáze: | OpenAIRE |
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