Nodal Stability Determines Signaling Range
| Autor: | Nadav Ben-Haim, Séverine Beck, Antonio J. Giraldez, Daniel B. Constam, Yu Chen, Katherine Joubin, Alexander F. Schier, J. Ann Le Good |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Mesoderm
animal structures Nodal Protein Immunoblotting Molecular Sequence Data Gene Expression Nodal signaling Biology Transfection General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences Paracrine signalling Tissue culture 0302 clinical medicine Transforming Growth Factor beta Chlorocebus aethiops Paracrine Communication medicine Animals Humans Amino Acid Sequence Autocrine signalling Zebrafish Body Patterning DNA Primers 030304 developmental biology 0303 health sciences Agricultural and Biological Sciences(all) Biochemistry Genetics and Molecular Biology(all) Lefty Sequence Analysis DNA Protein Structure Tertiary Cell biology Autocrine Communication medicine.anatomical_structure Mutagenesis COS Cells Endoderm General Agricultural and Biological Sciences NODAL Sequence Alignment 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Current Biology. (1):31-36 |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2004.12.062 |
| Popis: | Secreted TGFβ proteins of the Nodal family pattern the vertebrate body axes and induce mesoderm and endoderm [1]. Nodal proteins can act as morphogens [2], but the mechanisms regulating their activity and signaling range are poorly understood. In particular, it has been unclear how inefficient processing or rapid turnover of the Nodal protein influences autocrine and paracrine signaling properties [3, 4]. Here, we evaluate the role of Nodal processing and stability in tissue culture and zebrafish embryos. Removal of the pro domain potentiates autocrine signaling but reduces Nodal stability and signaling range. Insertion of an N-glycosylation site present in several related TGFβ proteins increases the stability of mature Nodal. The stabilized form of Nodal acts at a longer range than the wild-type form. These results suggest that increased proteolytic maturation of Nodal potentiates autocrine signaling, whereas increased Nodal stability extends paracrine signaling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|