Construction and immunogenicity analysis of nanoparticulated conjugate of heat-stable enterotoxin (STa) of enterotoxigenic Escherichia coli
| Autor: | Rihab Osman, Maha Nasr, Nasr-Eldin M. Aref |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030106 microbiology Bacterial Toxins macromolecular substances Biodegradable Plastics medicine.disease_cause digestive system Biochemistry Microbiology 03 medical and health sciences chemistry.chemical_compound Enterotoxins Mice Polylactic Acid-Polyglycolic Acid Copolymer Structural Biology Enterotoxigenic Escherichia coli medicine Heat-stable enterotoxin Animals Humans MTT assay Lactic Acid Neutralizing antibody Molecular Biology Escherichia coli Infections Carbodiimide biology Immunogenicity Escherichia coli Proteins technology industry and agriculture General Medicine Molecular biology Antibodies Bacterial Antibodies Neutralizing PLGA 030104 developmental biology chemistry biology.protein Nanoparticles Immunization Polyglycolic Acid Conjugate |
| Zdroj: | International journal of biological macromolecules. 106 |
| ISSN: | 1879-0003 |
| Popis: | The ultimate goal of this research was to overcome the low immunogenicity of the biological macromolecule (heat stable enterotoxin STa) via its conjugation to biodegradable PLGA nanoparticles (NP). STa was first isolated from Enterotoxigenic Escherichia coli (ETEC), purified and identified using reported HPLC procedures. Optimized homogenous PLGA NP, prepared using the nanoprecipitation technique were used for conjugating STa using the carbodiimide synthesis. Covalent binding of STa to PLGA NP was confirmed via FTIR and 1HNMR analysis. Safety and tolerability of the developed nanoparticulated STa-PLGA conjugate were confirmed by MTT assay on A549 lung cancer cells. After subcutaneous immunization, STA-PLGA NP conjugate induced a significant immune response in mice showing a strong binding and neutralizing antibody titer. The developed novel STa-PLGA NP conjugate is expected to provide promising protection against enterotoxigenic Escherichia coli (ETEC). |
| Databáze: | OpenAIRE |
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