Phytol is lethal for Amacr-deficient mice
| Autor: | J. Kalervo Hiltunen, Ari-Pekka Kvist, Remya R. Nair, Werner Schmitz, Kaija J. Autio, Myriam Baes, Eija M. Selkälä |
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| Rok vydání: | 2015 |
| Předmět: |
Pristanic acid
medicine.medical_specialty Phytanic acid Kupffer Cells Metabolite Racemases and Epimerases Biology Alpha-methylacyl-CoA racemase Kidney Bile Acids and Salts chemistry.chemical_compound Phytol Mice Internal medicine medicine Animals Molecular Biology Mice Knockout Kupffer cell Brain Cell Biology Peroxisome medicine.disease 3. Good health Endocrinology medicine.anatomical_structure Refsum disease chemistry Gene Expression Regulation Chemical and Drug Induced Liver Injury |
| Zdroj: | Biochimica et biophysica acta. 1851(10) |
| ISSN: | 0006-3002 |
| Popis: | α-Methylacyl-CoA racemase (Amacr) catalyzes the racemization of the 25-methyl group in C27-intermediates in bile acid synthesis and in methyl-branched fatty acids such as pristanic acid, a metabolite derived from phytol. Consequently, patients with Amacr deficiency accumulate C27-bile acid intermediates, pristanic and phytanic acid and display sensorimotor neuropathy, seizures and relapsing encephalopathy. In contrast to humans, Amacr-deficient mice are clinically symptomless on a standard laboratory diet, but failed to thrive when fed phytol-enriched chow. In this study, the effect and the mechanisms behind the development of the phytol-feeding associated disease state in Amacr-deficient mice were investigated. All Amacr-/- mice died within 36weeks on a phytol diet, while wild-type mice survived. Liver failure was the main cause of death accompanied by kidney and brain abnormalities. Histological analysis of liver showed inflammation, fibrotic and necrotic changes, Kupffer cell proliferation and fatty changes in hepatocytes, and serum analysis confirmed the hepatic disease. Pristanic and phytanic acids accumulated in livers of Amacr-/- mice after a phytol diet. Microarray analysis also revealed changes in the expression levels of numerous genes in wild-type mouse livers after two weeks of the phytol diet compared to a control diet. This indicates that detoxification of phytol metabolites in liver is accompanied by activation of multiple pathways at the molecular level and Amacr-/- mice are not able to respond adequately. Phytol causes primary failure in liver leading to death of Amacr-/- mice thus emphasizing the indispensable role of Amacr in detoxification of α-methyl-branched fatty acids. |
| Databáze: | OpenAIRE |
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