Hepatitis C virus NS5A protein increases hepatic lipid accumulation via induction of activation and expression of PPARgamma
| Autor: | Eunsin Ha, JaeHun Cheong, Jin Young Park, Kook Hwan Kim, Kyeong Jin Kim, Naoya Sakamoto |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Transcriptional Activation
PPARγ viruses Hepatitis C virus Biophysics Lipid accumulation Viral Nonstructural Proteins Biology NS5A medicine.disease_cause Biochemistry Cell Line Rosiglitazone PGC-1 Structural Biology RNA interference Gene expression Genetics medicine Animals Humans Hypoglycemic Agents Molecular Biology Heat-Shock Proteins Regulation of gene expression virus diseases Lipid metabolism Cell Biology biochemical phenomena metabolism and nutrition Lipid Metabolism medicine.disease Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha digestive system diseases PPAR gamma Gene Expression Regulation Liver Hepatic stellate cell Cancer research RNA Interference Thiazolidinediones Steatosis Transcription Factors |
| Zdroj: | FEBS Letters. 583:2720-2726 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2009.07.034 |
| Popis: | Steatosis is an established risk factor for disease progression in cases of chronic hepatitis C. Recently it was demonstrated that Hepatitis C virus (HCV) core and non-structural (NS) 2 proteins (NS2) induce lipid accumulation in hepatic cells. However, it has yet to be determined whether other HCV proteins are associated with lipid metabolism. The NS5A augmented the transcriptional activity and gene expression of PPARγ. Furthermore, NS5A increased the ability to recruit the transcriptional coactivator PGC-1s to the PPRE with PPARγ, as well as the interaction with PPARγ2 and PGC-1α. Our results indicate that NS5A may exploit multiple strategies that enhance PPARγ-induced lipid accumulation.Structured summaryMINT-7229685: PPAR gamma 2 (uniprotkb:P37231-2) physically interacts (MI:0914) with PGC1 alpha (uniprotkb:Q9UBK2) by pull down (MI:0096)MINT-7229712: PPAR gamma 2 (uniprotkb:P37231-2) physically interacts (MI:0914) with NS5A (uniprotkb:P26662) by pull down (MI:0096)MINT-7229698: PPAR gamma 2 (uniprotkb:P37231-2) physically interacts (MI:0914) with PGC1 alpha (uniprotkb:Q9UBK2) by anti tag coimmunoprecipitation (MI:0007)MINT-7229731: PPAR gamma 2 (uniprotkb:P37231-2) physically interacts (MI:0914) with NS5A (uniprotkb:P26662) by anti tag coimmunoprecipitation (MI:0007) |
| Databáze: | OpenAIRE |
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