Is Tracheal Transplantation Possible With Cryopreserved Tracheal Allograft and Hyperbaric Oxygen Therapy? An Experimental Study

Autor: Turgut Isitmangil, Orhan Yucel, Aptullah Haholu, Akin Yildizhan, Ali Memiş, Hakan Ay, Fatih Candas, Rauf Gorur
Rok vydání: 2016
Předmět:
Graft Rejection
Pulmonary and Respiratory Medicine
medicine.medical_specialty
Neovascularization
Physiologic
Lumen (anatomy)
030204 cardiovascular system & hematology
Risk Assessment
Sensitivity and Specificity
Statistics
Nonparametric
Organ transplantation
Neovascularization
Random Allocation
03 medical and health sciences
0302 clinical medicine
Fibrosis
Biopsy
medicine
Animals
Transplantation
Homologous
Rats
Wistar
Cryopreservation
Hyperbaric Oxygenation
medicine.diagnostic_test
business.industry
Biopsy
Needle
Graft Survival
Organ Transplantation
medicine.disease
Immunohistochemistry
Rats
Surgery
Trachea
Transplantation
Disease Models
Animal
medicine.anatomical_structure
030228 respiratory system
medicine.symptom
Cardiology and Cardiovascular Medicine
Wound healing
business
Respiratory tract
Zdroj: The Annals of Thoracic Surgery. 101:1139-1144
ISSN: 0003-4975
Popis: Background Allografts have achieved prominence for tracheal reconstruction because of their natural physiologic and anatomic structure, which preserves respiratory tract flexibility and lumen patency. The immunomodulatory effects of cryopreservation prevent tracheal allograft rejection. In addition, hyperbaric oxygen therapy (HBOT) accelerates wound healing by promoting epithelization and neovascularization. This experimental study investigated the early and late effects of HBOT on cryopreserved tracheal allografts (CTAs). Methods The study used 33 outbred Wistar rats weighing 300 to 350 g as allograft transplantation donors and recipients. Among these, 22 recipient rats were randomly assigned to the HBOT (n = 11) and control (n = 11) groups. Rats in the HBOT group were treated with 100% oxygen for 60 minutes at 2.5 atmospheres of absolute pressure for 7 days. Recipient rats in both groups were euthanized at 1 week (n = 5) and 4 weeks (n = 6) after transplantation, defined as the early and late periods, respectively. Results In the early period, no significant histopathologic differences were observed between groups ( p > 0.05). However, microscopic evaluation of the control group during the late period showed low epithelization of the CTA. In contrast, microscopic evaluation of the HBOT group during this same period revealed epithelium covering the transplanted CTA lumen. Significant epithelization and vascularization and significantly reduced inflammation and fibrosis were found in the HBOT group compared with the control group ( p Conclusions HBOT may be effective in tracheal reconstruction by increasing epithelization and neovascularization after extended tracheal resection. HBOT, therefore, should be considered in CTA transplantation.
Databáze: OpenAIRE
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