PD1 and PDL1 molecules control suppressor activity of regulatory T cells in chronic Chagas cardiomyopathy patients
| Autor: | Juliana A. S. Gomes, Giovane Rodrigo Sousa, Marcos Paulo Damásio, Nayara I. Medeiros, Manoel Otávio da Costa Rocha, Fernanda Fortes de Araújo, Rodrigo Correa-Oliveira, Rafaelle C. G. Fares-Gusmão, Walderez O. Dutra, Ana Thereza Chaves, Karine Silvestre Ferreira |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Cardiomyopathy Dilated Chagas Cardiomyopathy Male 0301 basic medicine Chagas disease Trypanosoma cruzi CD14 Programmed Cell Death 1 Receptor Immunology Cell Antigens Protozoan Apoptosis Caspase 3 T-Lymphocytes Regulatory B7-H1 Antigen 03 medical and health sciences 0302 clinical medicine Immune system Humans Immunology and Allergy Medicine Serologic Tests Aged biology business.industry Apyrase Interleukin-2 Receptor alpha Subunit Forkhead Transcription Factors General Medicine Middle Aged biology.organism_classification Fas receptor medicine.disease 030104 developmental biology medicine.anatomical_structure CD4 Antigens Female business 030215 immunology |
| Zdroj: | Human Immunology. 80:517-522 |
| ISSN: | 0198-8859 |
| Popis: | Introduction Chagas disease, caused by the protozoan Trypanosoma cruzi (T. cruzi), is the fourth most important tropical disease, which affects approximately 7 million people worldwide. The mechanisms involved in the development of this disease are not completely well understood. An important protective role of regulatory T cells (Treg) in Chagas disease has been observed; however, the specific mechanisms remain unclear. We evaluated apoptosis as a possible mechanism mediated by Treg cells (CD4+CD25HighFOXP3+) to orchestrate the immune response in chronic Chagas disease. Methods and results Patients with Chagas disease were grouped as the indeterminate (IND; asymptomatic patients with Chagas disease; n = 10) and dilated cardiomyopathy (CARD; n = 10). Healthy T. cruzi-negative individuals (NI; n = 10) were included as a control group. In order to evaluate the apoptotic cell profile, the expression of PD1, PD1L, CD39, CD95, CD95L molecules were investigated. We also evaluated the proportion of CD14+ cells expressing caspase 3. The IND group presented a substantially higher expression of CD39 by Treg cells as compared to the CARD group. On the other hand, the CARD group showed higher expression of PD-1 by Treg cells than both NI and IND groups. Significant positive correlations were observed between Treg CD95L+ cells and CD14 cells expressing caspase 3 as well as between Treg CD39 cells and CD14+ Caspase3+ cells in the IND group. Conclusion Our data indicate that the expressions of different molecules that induce apoptosis are associated with suppressive mechanisms mediated by Treg cells and suggest a possible role for PD1 and PDL1 molecules in the morbidity of chronic Chagas disease. |
| Databáze: | OpenAIRE |
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