Serotonin and Melatonin Do Not Play a Prominent Role in the Growth of Prostate Cancer Cell Lines
| Autor: | Oliver W. Hakenberg, Axel Meye, Manfred P. Wirth, I. Pirozhok, Susanne Fuessel |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Serotonin medicine.medical_specialty Cell Survival Urology Apoptosis Pharmacology Biology urologic and male genital diseases DU145 Cell Line Tumor Internal medicine LNCaP medicine Humans RNA Messenger ddc:610 Receptor 5-HT receptor Cell Proliferation Melatonin Prostate cancer cell lines Serotonin Melatonin Cellular viability Apoptosis Cell cycle Dose-Response Relationship Drug Cell Cycle Prostatic Neoplasms Cell cycle Prostatakrebs Prostata-Krebs-Zelllinien Serotonin Melatonin Cellular viability Apoptose Zellzyklus Serotonin Receptor Agonists Endocrinology Cell culture Receptor Serotonin 5-HT1A Receptor Serotonin 5-HT1B Serotonin Antagonists |
| Zdroj: | Urol Int 2010;84:452–460, ISSN: 0042-1138 |
| Popis: | Objectives: To investigate the effects of serotonin and melatonin (MLT) on the regulation of malignant growth and the activity of serotonin receptors (5HTR1a/-1b) in prostate cancer (PCa) cell lines. Materials and Methods: In four PCa cell lines (LNCaP, 22RV1, PC3, DU145) and two reference cell lines 5HTR1a and -1b, relative mRNA expression levels were assessed. Different serotonin and MLT receptor agonists and antagonists were used in stimulation and inhibition experiments. Results: mRNA expression of 5HTR1b was higher than that of 5HTR1a in all PCa cell lines. Serotonin showed a significant growth stimulatory effect in all PCa lines. The 5HTR1a and -1b agonists/antagonists did not significantly affect viability. MLT inhibited viability only in PC3 cells. Similarly, the 5HTR1a antagonist induced apoptotic changes in PC3 cells only at 10–4M, while the 5HTR1b antagonist induced necrosis at 10–4M in all cell lines. Cell cycle alterations were seen in PC3 and DU145 cells under the influence of the 5HTR1a antagonist. Conclusions: Serotonin receptor antagonists and agonists as well as MLT influence viability and apoptosis of PCa cell lines at supraphysiologic concentrations. In contrast to other reports, our results do not support a regulatory role of serotonin or MLT receptor activation or inhibition in PCa growth. |
| Databáze: | OpenAIRE |
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