Insulin-like growth factor-I and its receptor in neovascular age-related macular degeneration
| Autor: | Karel H. M. van Wely, Antoinette C Lambooij, Cornelia M. Mooy, Dicky J Lindenbergh-Kortleve, Robert W A M Kuijpers, Mike Kliffen |
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| Přispěvatelé: | Ophthalmology, Pathology, Surgical clinical sciences |
| Rok vydání: | 2003 |
| Předmět: |
Male
Retinal Ganglion Cells genetic structures Retinal Ganglion Cells/metabolism medicine.medical_treatment RNA Messenger/metabolism Choroid/blood supply Receptor IGF Type 1 Neovascularization Immunoenzyme Techniques Macular Degeneration Diabetic Retinopathy/metabolism Insulin-Like Growth Factor I Pigment Epithelium of Eye In Situ Hybridization Medicine(all) Aged 80 and over Middle Aged Choroidal neovascularization medicine.anatomical_structure Female medicine.symptom Retinopathy Adult medicine.medical_specialty Endothelium Biology Pigment Epithelium of Eye/metabolism Internal medicine Ophthalmology medicine Humans RNA Messenger Aged Choroidal Neovascularization/etiology Retina Retinal pigment epithelium Diabetic Retinopathy Receptor IGF Type 1/genetics Choroid Growth factor Retinal Vessels/metabolism Retinal Vessels RNA Probes Macular degeneration medicine.disease eye diseases Choroidal Neovascularization Endocrinology Macular Degeneration/complications sense organs Endothelium Vascular Insulin-Like Growth Factor I/genetics Endothelium Vascular/metabolism |
| Zdroj: | Investigative Ophthalmology & Visual Science, 44, 2192-2198. Association for Research in Vision and Ophthalmology Inc. |
| ISSN: | 0146-0404 |
| Popis: | PURPOSE. The insulin-like growth factor (IGF)-I protein is a growth-promoting polypeptide that can act as an angiogenic agent in the eye. The purpose of the current study was to localize the expression of IGF-I and its receptor (IGF-IR) mRNA and IGF-IR protein in situ in the normal human eye and to examine the presence of expression in eyes with neovascular age-related macular degeneration (AMD). METHODS. Formalin-fixed, paraffin-embedded slides of 4 normal control eyes and 14 eyes with choroidal neovascularization (CNV) secondary to AMD were examined. Three eyes with proliferative diabetic retinopathy were studied as the positive control. IGF-I and IGF-IR mRNA was detected by in situ hybridization with digoxigenin-labeled RNA probes. IGF-IR protein was studied by immunohistochemistry. RESULTS. In the normal retina, IGF-I and IGF-IR mRNA expression was found throughout the neuroretinal layers, in the retinal pigment epithelium (RPE), and in some choriocapillary and retinal capillary endothelial cells. In eyes with CNV we found IGF and IGF-IR mRNA in capillary endothelial cells, some transdifferentiated RPE, and fibroblast-like cells. IGF-IR protein was found in normal eyes in all neuroretinal layers, in the RPE, and in the choroidal vessels. In eyes with CNV, IGF-IR protein was present in the RPE monolayer, in transdifferentiated RPE, and in newly formed vessels. CONCLUSIONS. The colocalization of protein and receptor indicates an autocrine function of IGF-I in the normal human retina. Because IGF-I participates in ocular neovascularization, synthesis of IGF-IR and IGF-I in endothelial cells, RPE cells, and fibroblast-like cells in CNV may point toward a role for this growth factor in the pathogenesis of neovascular AMD. (Invest Ophthalmol Vis Sci. 2003;44:2192‐2198) DOI:10.1167/ iovs.02-0410 |
| Databáze: | OpenAIRE |
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