Ultrasound microbubble-carried PNA targeting to c-myc mRNA inhibits the proliferation of rabbit iliac arterious smooth muscle cells and intimal hyperplasia
| Autor: | Yue-Xuan Chen, Da-Fang Wu, Wen-Ping Ling, Xiao-Ling Chen, Xia He, Jun Ji |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Intimal hyperplasia Myocytes Smooth Muscle Cell Pharmaceutical Science 02 engineering and technology In situ hybridization Transfection Iliac Artery 03 medical and health sciences 0302 clinical medicine medicine Animals Ultrasonics RNA Messenger Cell Proliferation Hyperplasia Microbubbles biology business.industry Ultrasound Albumin General Medicine 021001 nanoscience & nanotechnology medicine.disease Molecular biology Proliferating cell nuclear antigen medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Nanoparticles Rabbits 0210 nano-technology business |
| Zdroj: | Drug Delivery. 23:2482-2487 |
| ISSN: | 1521-0464 1071-7544 |
| DOI: | 10.3109/10717544.2015.1014947 |
| Popis: | To elucidate the transfected effect of albumin ultrasound microbubbles carrying peptide nucleic acids (PNAs) against c-myc gene to the vascular walls and their effect on the intimal proliferation induced by vascular denudation.A rabbit iliac artery intimal proliferation model was constructed and PNA against c-myc mRNA was designed and synthesized and was added to albumin solution before ultrasound microbubbles were prepared and encapsulated in matrix of albumin. The ultrasound microbubbles carrying PNA were transfected to intima under ultrasound exposure. The transfected effect was identified by a histochemical method and the expression of c-myc was detected by in situ hybridization. The proliferation of intimal smooth muscle cells was estimated by the expression of proliferative cell nuclear antigen (PCNA) of them. The intimal area and thickness were judged morphologically for intimal hyperplasia.The ultrasound microbubbles with PNA were successfully prepared and c-myc PNA was transfected to vascular intimal cells. The expression of c-myc and PCNA by intimal vascular smooth muscle cells (vSMCs) was inhibited significantly and the intimal thickness and area were reduced remarkably.Transfection of c-myc PNA could inhibit proliferartion of vSMCs and intima in the rabbit iliac artery intimal proliferation model and the targeted transfection of albumin ultrasound microbubbles carrying PNA offers a feasible way to facilitate its access to specific cells in vivo and produce bioavailability. |
| Databáze: | OpenAIRE |
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