Functional link between mitochondria and Rnr3, the minor catalytic subunit of yeast ribonucleotide reductase
| Autor: | Charles Boone, Michael Costanzo, Isaac Corcoles-Saez, Jean-Luc Ferat, Rita S. Cha |
|---|---|
| Přispěvatelé: | Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Evolution et maintenance des chromosomes circulaires (EMC2), Département Biologie des Génomes (DBG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0209 industrial biotechnology
autophagy DNA damage kinase Protein subunit [SDV]Life Sciences [q-bio] Saccharomyces cerevisiae 02 engineering and technology dna-damage response Mitochondrion Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology Applied Microbiology and Biotechnology 020901 industrial engineering & automation checkpoint atr Virology 0202 electrical engineering electronic engineering information engineering Genetics Translocase Mec1 gene Rnr1 Molecular Biology Gene lcsh:QH301-705.5 Rnr3 biology Chemistry 020208 electrical & electronic engineering DNA replication Cell Biology stability biology.organism_classification Cell biology dNTP mitochondria Ribonucleotide reductase cell-cycle lcsh:Biology (General) carbon source biology.protein Parasitology progression respiration Research Article |
| Zdroj: | Microbial Cell, Vol 6, Iss 6, Pp 286-294 (2019) Microbial Cell Microbial Cell (Graz, Austria) Microbial Cell (Graz, Austria), 2019, 6 (6), pp.286--294. ⟨10.15698/mic2019.06.680⟩ |
| ISSN: | 2311-2638 |
| Popis: | International audience; Ribonucleotide reductase (RNR) is an essential holoenzyme required for de novo synthesis of dNTPs. The Saccharomyces cerevisiae genome encodes for two catalytic subunits, Rnr1 and Rnr3. While Rnr1 is required for DNA replication and DNA damage repair, the function(s) of Rnr3 is unknown. Here, we show that carbon source, an essential nutrient, impacts Rnr1 and Rnr3 abundance: Non-fermentable carbon sources or limiting concentrations of glucose down regulate Rnr1 and induce Rnr3 expression. Oppositely, abundant glucose induces Rnr1 expression and down regulates Rnr3. The carbon source dependent regulation of Rnr3 is mediated by Mec1, the budding yeast ATM/ATR checkpoint response kinase. Unexpectedly, this regulation is independent of all currently known components of the Mec1 DNA damage response network, including Rad53, Dun1, and Tel1, implicating a novel Mec1 signalling axis. rnr3Δ leads to growth defects under respiratory conditions and rescues temperature sensitivity conferred by the absence of Tom6, a component of the mitochondrial TOM (translocase of outer membrane) complex responsible for mitochondrial protein import. Together, these results unveil involvement of Rnr3 in mitochondrial functions and Mec1 in mediating the carbon source dependent regulation of Rnr3. |
| Databáze: | OpenAIRE |
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