Antagonistic role of vitamin D3 and retinoic acid on the differentiation of chicken hematopoietic macrophages into osteoclast precursor cells
| Autor: | P. Jurdic, E. Lazarides, F. Solari, Olivier Gandrillon, C. Woods, C. Domenget |
|---|---|
| Přispěvatelé: | Unité mixte de recherche biologie moléculaire de la cellule, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Laviron, Nathalie, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Receptors Retinoic Acid Molecular Sequence Data Retinoic acid Osteoclasts Tretinoin Biology Giant Cells 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Osteoclast Internal medicine medicine Macrophage Animals Progenitor cell [SDV.BC] Life Sciences [q-bio]/Cellular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Carbonic Anhydrases Cholecalciferol Yolk Sac [SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Cell Nucleus 0303 health sciences Blood Cells Base Sequence Macrophages Stem Cells Cell Differentiation [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism Hematopoietic Stem Cells Cell biology Haematopoiesis medicine.anatomical_structure Biochemistry chemistry Giant cell 030220 oncology & carcinogenesis Oligonucleotide Probes Macrophage proliferation Chickens medicine.drug |
| Zdroj: | Endocrinology Endocrinology, Endocrine Society, 1995, 136 (1), pp.85-95 Endocrinology, 1995, 136 (1), pp.85-95 |
| ISSN: | 0013-7227 |
| Popis: | An in vitro culture model of osteoclast differentiation is described which is derived from homogeneous populations of chick yolk sac and peripheral blood macrophages. In primary cultures, both types of macrophages undergo a proliferative phase, become quiescent after reaching high cell densities, then aggregate and eventually form large multinucleated giant cells (MNGCs), presumably by fusion. These MNGCs can be characterized as premature osteoclasts on the basis of several morphological and biochemical criteria, although they do not undergo the final differentiation step rendering them competent to resorb bone in vitro. Clonal analysis of single cell-derived colonies indicates that all macrophages have the potential to differentiate into these osteoclast-like cells under these culture conditions. Both retinoic acid and 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] modulate macrophage growth, but in an antagonistic manner. Although retinoic acid strongly promotes macrophage proliferation and impedes MNGC formation, 1,25-(OH)2D3 inhibits proliferation and changes the kinetics of MNGC formation. Combination experiments reveal that the proliferative signals induced by retinoic acid can override the signal to differentiate induced by 1,25-(OH)2D3. Our results indicate that even though retinoic acid and vitamin D3 act through homologous receptors, they have dramatically opposing effects on macrophage differentiation toward osteoclast progenitors. |
| Databáze: | OpenAIRE |
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