Tumor necrosis factor-alpha production by human islets leads to postisolation cell death
| Autor: | Jean Tchervenkov, Stephen C. Hanley, Steven Paraskevas, S. Liu, Mark Lipsett, Mauro Castellarin, Lawrence Rosenberg |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Male endocrine system medicine.medical_specialty Programmed cell death Transcription Genetic medicine.medical_treatment Alpha (ethology) Apoptosis Biology Islets of Langerhans Internal medicine Insulin Secretion medicine Cadaver Humans Insulin Secretion Cells Cultured DNA Primers Transplantation geography geography.geographical_feature_category Cell Death Tumor Necrosis Factor-alpha Middle Aged Islet Tissue Donors Endocrinology Cytokine Gene Expression Regulation Tumor necrosis factor alpha Female Peptides |
| Zdroj: | Transplantation. 82(6) |
| ISSN: | 0041-1337 |
| Popis: | BACKGROUND Recent successes in islet transplantation highlight the importance of islet isolation by experienced centers and minimization of cell injury as crucial to the achievement of insulin independence. Islet injury may manifest as cell death by apoptosis, shorter graft survival, and the need for retransplantation. Although an inflammatory cytokine response at the graft site is known to inhibit engraftment, recent evidence indicates that islet cells may contribute to this response. METHODS Isolated human islets were cultured for up to one week in serum-free CMRL-1066 with 25 microM of tumor necrosis factor (TNF)alpha inhibitor RDP58. Gene expression was measured by reverse transcriptase polymerase chain reaction, apoptosis and TNFalpha secretion by enzyme-linked immunosorbent assay and enzyme-linked immunospot, and islet function by stimulated insulin secretion. RESULTS Isolation induced a twofold increase in TNFalpha expression between days one and three (P |
| Databáze: | OpenAIRE |
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