Packaging limits and stability of HIV-1 sequences in a coxsackievirus B vector
| Autor: | Hwee L. Ng, Paul Krogstad, John P. Miller, Yongzhi Geng, Otto O. Yang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Sexual transmission
viruses Genetic Vectors Biology Coxsackievirus gag Gene Products Human Immunodeficiency Virus Genomic Instability Article Epitope Virus Cell Line law.invention law Humans Vector (molecular biology) Codon Base Composition General Veterinary General Immunology and Microbiology Virus Assembly Public Health Environmental and Occupational Health virus diseases Group-specific antigen biology.organism_classification Virology Molecular biology Enterovirus B Human Infectious Diseases Capsid HIV-1 Recombinant DNA Molecular Medicine |
| Zdroj: | Vaccine. 27:3992-4000 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2009.04.035 |
| Popis: | Enteroviruses elicit protective mucosal immune responses that could be harnessed as part of a strategy to prevent sexual transmission of the human immunodeficiency virus-1 (HIV-1). We report the construction of replication competent recombinant vectors of coxsackievirus B3 (CVB3) that express one or more portions of the HIV-1 Gag protein. Vectors containing the capsid domain of Gag were initially genetically unstable with protein expression lost after brief passage in tissue culture. Codon modification to increase the G/C content of the HIV-1 capsid sequence resulted in enhanced genetic stability of CVB3 vectors during in vitro passage. Cells infected with a vector expressing the matrix (MA) subunit of the HIV-1 Gag protein were susceptible to lysis by CD8 T cell clones specific for the SL9 epitope found within MA. These studies suggest that CVB3 vectors may be useful as vaccine vector candidates, if hurdles in class I antigen presentation and stability can be overcome. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect