Bio-functionalized silk hydrogel microfluidic systems
| Autor: | Matthew B. Applegate, Jeannine M. Coburn, Siwei Zhao, Peter Tseng, Jodie E. Moreau, Anne S. Golding, Fiorenzo G. Omenetto, Benjamin P. Partlow, Ying Chen, David L. Kaplan |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male food.ingredient Materials science Optical Phenomena Microfluidics Biophysics Silk Fibroin Bioengineering Nanotechnology Biocompatible Materials 02 engineering and technology Gelatin Hydrogel Polyethylene Glycol Dimethacrylate Biomaterials 03 medical and health sciences chemistry.chemical_compound food Tissue engineering Human Umbilical Vein Endothelial Cells Animals Humans Microchannel Polydimethylsiloxane 021001 nanoscience & nanotechnology 030104 developmental biology SILK chemistry Mechanics of Materials Self-healing hydrogels Ceramics and Composites 0210 nano-technology |
| Zdroj: | Biomaterials. 93 |
| ISSN: | 1878-5905 |
| Popis: | Bio-functionalized microfluidic systems were developed based on a silk protein hydrogel elastomeric materials. A facile multilayer fabrication method using gelatin sacrificial molding and layer-by-layer assembly was implemented to construct interconnected, three dimensional (3D) microchannel networks in silk hydrogels at 100 μm minimum feature resolution. Mechanically activated valves were implemented to demonstrate pneumatic control of microflow. The silk hydrogel microfluidics exhibit controllable mechanical properties, long-term stability in various environmental conditions, tunable in vitro and in vivo degradability in addition to optical transparency, providing unique features for cell/tissue-related applications than conventional polydimethylsiloxane (PDMS) and existing hydrogel-based microfluidic options. As demonstrated in the work here, the all aqueous-based fabrication process at ambient conditions enabled the incorporation of active biological substances in the bulk phase of these new silk microfluidic systems during device fabrication, including enzymes and living cells, which are able to interact with the fluid flow in the microchannels. These silk hydrogel-based microfluidic systems offer new opportunities in engineering active diagnostic devices, tissues and organs that could be integrated in vivo, and for on-chip cell sensing systems. |
| Databáze: | OpenAIRE |
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