Puerarin inhibits vascular calcification of uremic rats
| Autor: | Hailin Liu, Xiuli Zhang, Xinglong Zhong, Caiwen Ou, Zehua Li, Shao-Ai Cai, Minsheng Chen, Pingzhen Yang |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Vascular smooth muscle Anti-Inflammatory Agents Inflammation Muscle Smooth Vascular Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Puerarin In vivo Internal medicine medicine Animals Vascular Calcification Uremia Pharmacology chemistry.chemical_classification Reactive oxygen species Chemistry medicine.disease Isoflavones In vitro Rats 030104 developmental biology Endocrinology Tumor necrosis factor alpha medicine.symptom 030217 neurology & neurosurgery Calcification |
| Zdroj: | European journal of pharmacology. 855 |
| ISSN: | 1879-0712 |
| Popis: | The risk of cardiovascular events in patients with chronic kidney disease is tremendously increased due to vascular calcification. Local vascular inflammation significantly promotes vascular calcification. A previous study has shown that puerarin inhibits calcification of mouse vascular smooth muscle cells (VSMCs) in vitro, but whether puerarin can inhibit vascular calcification in vivo and the underlying mechanisms remain unclear. In this study, we found that rat VSMCs treated with calcifying medium showed more mineral deposition, and this effect was inhibited by puerarin in a dose-dependent manner, as detected by alizarin red staining and calcium content assay. Puerarin also prevented the trans-differentiation of VSMCs into osteoblast-like cells, indicated by down-regulation of bone-related genes including Runx2 and BMP2. In vivo study of uremic rats induced by renal nephrectomy further confirmed the inhibitory effect of puerarin on vascular calcification in uremic rats. Puerarin treatment significantly prevented mineral deposition in rat aortas and down-regulated the expression of Runx2 and BMP2. Furthermore, we detected the levels of pro-inflammatory cytokines including IL-1β, IL-6, IL-18 and TNFα in vitro and in vivo. The levels of IL-1β were remarkably increased in both calcified VSMCs and aortas of uremic rats, while puerarin treatment markedly decreased the expression of IL-1β. In addition, we found that puerarin reduced IL-1β possibly through targeting NLRP3/Caspase1/IL-1β and NF-κB signaling pathways and inhibiting the generation of reactive oxygen species. Taken together, we demonstrated that puerarin has the capability of inhibiting vascular calcification in uremic rats by inhibiting inflammation. |
| Databáze: | OpenAIRE |
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