Cysteine-dependent immune regulation by TRX and MIF/GIF family proteins
| Autor: | Toshinori Nakayama, Junji Yodoi, Yasuyuki Ishii, Junko Sakakura-Nishiyama, Yong-Won Kwon, Yumiko Nishinaka, Norihiko Kondo, Aoi Son, Masaki Tanito, Masaru Taniguchi, Yoshiyuki Matsuo |
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| Rok vydání: | 2004 |
| Předmět: |
animal structures
T-Lymphocytes viruses medicine.medical_treatment T cell Immunology Apoptosis Biology Jurkat cells Proinflammatory cytokine Jurkat Cells Mice Thioredoxins medicine Animals Humans Immunology and Allergy Cysteine Macrophage Migration-Inhibitory Factors Lymphokines fungi Hydrogen Peroxide Transfection Molecular biology Intramolecular Oxidoreductases Cytokine medicine.anatomical_structure Cell culture Multigene Family embryonic structures Thioredoxin |
| Zdroj: | Immunology Letters. 92:143-147 |
| ISSN: | 0165-2478 |
| Popis: | Thioredoxin (TRX) superfamily proteins that contain a conserved redox-active site -Cys-Xa.a.-Xa.a.-Cys- includes proinflammatory cytokine, macrophage migration inhibiting factor (MIF) and the immune regulatory cytokine, glycosylation inhibiting factor (GIF) in which Cys-60 is cysteinylated. In this report, we have analyzed the functional interaction between TRX and MIF/GIF. The stable Jurkat T cell line transfected with human TRX gene (TRX-transfectant) was highly resistant to hydrogen peroxide-induced apoptosis, but not the cell line transfected with vector (mock-transfectant). The expression level of MIF/GIF protein of TRX-transfectant was lower than that of mock-transfectant. Conversely, the expression level of intracellular TRX protein in CD4(+)-T cells derived from MIF -/- mice were significantly higher than that from background BALB/c mice. These findings collectively suggest that oxidative stress-induced apoptosis on T lymphocytes might be protected by the reciprocal regulation of TRX and MIF/GIF expression. |
| Databáze: | OpenAIRE |
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