Human Dentin-matrix-derived Bone Morphogenetic Protein
| Autor: | J Matsumoto, Kazuhisa Bessho, Toshirou Tagawa, Mutsuo Murata, N Tanaka |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
0301 basic medicine
animal structures Molecular Sequence Data Matrix (biology) Bone morphogenetic protein 03 medical and health sciences 0302 clinical medicine In vivo Humans Amino Acid Sequence Growth Substances Immunoelectrophoresis General Dentistry Polyacrylamide gel electrophoresis Peptide sequence chemistry.chemical_classification Chemistry Proteins Bone morphogenetic protein 10 030206 dentistry Alkaline Phosphatase Molecular biology Amino acid 030104 developmental biology Biochemistry Bone Morphogenetic Proteins Dentin embryonic structures Alkaline phosphatase Electrophoresis Polyacrylamide Gel |
| Zdroj: | Journal of Dental Research. 70:171-175 |
| ISSN: | 1544-0591 0022-0345 |
| DOI: | 10.1177/00220345910700030301 |
| Popis: | Bone morphogenetic protein (BMP) was extracted from human dentin matrix with 4 mol/L guanidine-HCl and was purified by liquid chromatography. SDS-PAGE and IEF showed that the purified BMP was homogeneous and induced new bone formation in situ after three weeks when implanted into muscle pouches in Wistar rats. The molecular weight of BMP was estimated to be about 20.0 kDa by SDS-PAGE, and the pI value was 8.8 by IEF. Amino acid analysis suggested that BMP is a protein containing 191 amino acids. A partial amino acid sequence was obtained from the final purified BMP. Dentin-matrix-derived BMP is probably not identical to, but is similar to, bone-matrix-derived BMP, though both types of BMP have the same action in vivo. |
| Databáze: | OpenAIRE |
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