Polyamide Nanogels from Generally Recognized as Safe Components and Their Toxicity in Mouse Preimplantation Embryos
| Autor: | Jesse Mager, Priyaa Prasad, Mijanur Rahaman Molla, Mine Canakci, Wei Cui, Barbara A. Osborne, Sankaran Thayumanavan |
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| Rok vydání: | 2015 |
| Předmět: |
Polymers and Plastics
Cell Survival Nanogels Bioengineering Article Polyethylene Glycols Biomaterials HeLa Mice Generally recognized as safe Materials Chemistry medicine Animals Humans Polyethyleneimine Blastocyst A549 cell biology Chemistry HEK 293 cells Embryo biology.organism_classification Molecular biology In vitro Cell biology Nylons medicine.anatomical_structure HEK293 Cells embryonic structures MCF-7 Cells Female Hydrophobic and Hydrophilic Interactions Nanogel HeLa Cells |
| Zdroj: | Biomacromolecules. 16(11) |
| ISSN: | 1526-4602 |
| Popis: | Safe delivery systems that can not only encapsulate hydrophobic drug molecules, but also release them in response to specific triggers, are important in several therapeutic and biomedical applications. In this paper, we have designed a nanogel based on molecules that are generally recognized as safe (GRAS). We have shown that the resultant polymeric nanogels exhibit responsive molecular release, and also show high in vitro cellular viability on HEK 293T, HeLa, MCF 7 and A549 cell lines. The toxicity of these nanogels was further evaluated with a highly sensitive assay using mouse preimplantation embryo development, where blastocysts were formed after four days of in vitro culture and live pups were born when morulae/early blastocysts were transferred to the uteri of surrogate recipients. Our results indicate that these nanogels are non-toxic during mammalian development and do not alter normal growth or early embryo success rate. |
| Databáze: | OpenAIRE |
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