Nimodipine rescues N-methyl-N-nitrosourea-induced retinal degeneration in rats
| Autor: | Dan Wang, Yan Li, Gui-yuan Sun, Zhuo Wang, Qiu-hua Zhang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Retinal degeneration
Telomerase Antioxidant medicine.medical_treatment Pharmaceutical Science Retinal Glutathione Biology medicine.disease N-methyl-N-nitrosourea Molecular biology Reverse transcriptase chemistry.chemical_compound chemistry Drug Discovery retinal degeneration medicine N-Methyl-N-nitrosourea Original Article Nimodipine medicine.drug |
| Zdroj: | Pharmacognosy Magazine |
| ISSN: | 0973-1296 |
| DOI: | 10.4103/0973-1296.111276 |
| Popis: | Background: That nimodipine (NMD) is potentially useful for ophthalmic treatment. However, the effect of NMD is unknown on retinal degenerative diseases. Objective: The purpose of the present study was to investigate the effect of NMD on N-methyl-N-nitrosourea (MNU)-induced retinal degeneration (RD) and elucidate its possible mechanisms. Materials and Methods: Morphological observation of NMD on MNU-induced RD was evaluated by light microscopy and electron microscopy. Nonenzymatic antioxidant glutathione (GSH) was measured by a colorimetric method. Transforming growth factor-beta (TGF-β) was measured by enzyme-linked immunosorbent assay (ELISA). Telomerase was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results: The significantly protective effect of NMD on MNU-induced RD was demonstrated morphologically. NMD increased the content of GSH and decreased the level of TGF-β in rat retina. RT-PCR analysis demonstrated that NMD treatment significantly decreased mRNA level of telomerase. Conclusion: These data suggest that NMD inhibit MNU-induced RD in rats. The expressions of TGF-β, telomerase and GSH contents might partially contribute to its protective effects on MNU-induced RD. |
| Databáze: | OpenAIRE |
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