Lactobacillus gasseri K7 modulates the blood cell transcriptome of conventional mice infected with Escherichia coli O157:H7
| Autor: | Š. Špela, M. Černe, Bojana Bogovič Matijašić, Richard F. Hurrell, Irena Rogelj, F.M. Sagaya, Biljana Hacin, G. Vergères, Gorazd Tompa, A. Ihan |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Inflammation Escherichia coli O157 medicine.disease_cause Lactobacillus gasseri Applied Microbiology and Biotechnology Microbiology Blood cell Transcriptome Mice Immune system Gene expression medicine Animals Escherichia coli Escherichia coli Infections Subclinical infection Blood Cells biology Probiotics General Medicine biology.organism_classification Molecular biology Gastrointestinal Tract Mice Inbred C57BL Lactobacillus medicine.anatomical_structure Female medicine.symptom Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 116:1282-1296 |
| ISSN: | 1364-5072 |
| DOI: | 10.1111/jam.12440 |
| Popis: | Aims As the immune cells underlying the intestinal barrier sense luminal microbial signals, blood cell transcriptomics may identify subclinical changes triggered by gut bacteria that may otherwise not be detected. We have therefore investigated how Lactobacillus gasseri K7 and enterohemorrhagic Escherichia coli O157:H7 modulate the blood cell transcriptome of mice possessing an intact microbiota. Methods and Results We have analysed the transcriptome of five groups of C57BL/6J mice: (i) control, (ii) inoculated with a single dose of E. coli, (iii) inoculated during 2 weeks with Lact. gasseri, (iv) co-inoculated with E. coli and Lact. gasseri, (v) inoculated with Lact. gasseri prior to E. coli infection. The transcriptome could distinguish between the five treatment groups. Gene characteristics of bacterial infection, in particular inflammation, were upregulated in the mice inoculated with E. coli. Lact. gasseri had only mild effects on the transcriptome but modified the gene expression induced by E. coli. Conclusions The transcriptome differentiates mice inoculated orally with E. coli, Lact. gasseri and combinations of these two strains. Significance and Impact of the Study These results suggest that the blood cell transcriptome can be used as a source of biomarkers to monitor the impact of probiotics in subclinical models of infectious disease. |
| Databáze: | OpenAIRE |
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