Factors Contributing to False-Negative Enzyme-Linked Immunospot Assay for Interferon-Gamma Results in Active Tuberculosis
Autor: | Zhigang Liu, Hongyan Wu, Guoli Lian, Min He, Fangxiong Du |
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Rok vydání: | 2017 |
Předmět: |
medicine.medical_specialty
Enzyme-Linked Immunospot Assay Multivariate analysis Tuberculosis 030231 tropical medicine Extra pulmonary General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Interferon-gamma 0302 clinical medicine Internal medicine medicine Humans Interferon gamma Adult patients business.industry Active tuberculosis medicine.disease Clinical trial 030220 oncology & carcinogenesis Immunology Enzyme linked immunospot assay business Interferon-gamma Release Tests medicine.drug |
Zdroj: | Clinical laboratory. 63(4) |
ISSN: | 1433-6510 |
Popis: | Background The enzyme-linked immunospot assay test (T-SPOT.TB) is effective for detecting TB, but there are still some persons with tuberculosis (TB) who have negative results. The aim of this study was to analyze the factors that contribute to false negative results in active TB patients. Methods We investigated the clinical trial and laboratory data of the active TB patients hospitalized in the First Affiliated Hospital of Xi'an Jiaotong University from May 2013 to November 2014. Results In 556 active TB patients, 198 infected sites were pulmonary, 358 infected sites were extra pulmonary, and 13.85% (77/556) had false negative T SPOT.TB results. The univariate and multivariate analysis showed that age and bone/joint TB were the factors contributing to the T-SPOT. TB false negative results and pleural TB were related to true positive results. Conclusions The site of TB may significantly affect the sensitivity of T-SPOT.TB. False negative results of TSPOT.TB are associated with older age and bone/joint TB, so in older adult patients or those with bone/joint TB, the exclusion of TB should be regarded with caution when T-SPOT.TB results are negative. |
Databáze: | OpenAIRE |
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