Rat liver cytoplasmic ribonucleic acids: some aspects of their localization and isolation
| Autor: | Abraham Aronow, Tsuyoshi Kakefuda, C. Wesley Dingman |
|---|---|
| Rok vydání: | 1970 |
| Předmět: |
Electrophoresis
Male Cytoplasm Liver cytology Detergents Mitochondria Liver Mitochondrion Biology Tritium Biochemistry Genetics and Molecular Biology (miscellaneous) Ribosome Bile Acids and Salts Surface-Active Agents Animals Polyacrylamide gel electrophoresis Carbon Isotopes RNA Ribosomal RNA Molecular biology Rats Molecular Weight Microscopy Electron Biochemistry Liver Microsome Microsomes Liver Ribosomes |
| Zdroj: | Biochimica et biophysica acta. 224(1) |
| ISSN: | 0006-3002 |
| Popis: | Analytical fractionation (by polyacrylamide gel electrophoresis) of RNA from various cytoplasmic fractions of rat liver was used to examine the effect of certain isolation procedures on the integrity of RNA molecules. The removal of the cytoplasmic supernatant from microsomal fractions was shown to result in the subsequent partial degradation of rRNA's, particularly newly-synthesized species. Likewise, sodium deoxycholate treatment of microsomes resulted in the preferential release of newly-synthesized RNA and the preferential disruption of newly-synthesized ribosomes. Rapid isolation techniques allowed us to demonstrate that less than 20 % of the newly-synthesized cytoplasmic RNA is of heterogeneous size, the remainder being accounted for by the usual rRNA species and S RNA. Two species of RNA, peculiar to mitochondria, are described; mitochondria were also characterized by a large quantity of S RNA relative to the ribosomal species present. |
| Databáze: | OpenAIRE |
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