Role for the splicing factor TCERG1 in Cajal body integrity and snRNP assembly
| Autor: | Cristina Moreno-Castro, Silvia Prieto-Sánchez, Cristina Hernández-Munain, Carlos Suñé, Noemí Sánchez-Hernández |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Junta de Andalucía, European Commission, Hernández-Munaín, Cristina, Suñé, Carlos, Hernández-Munaín, Cristina [0000-0002-6058-2458], Suñé, Carlos [0000-0002-7991-0458] |
| Rok vydání: | 2019 |
| Předmět: |
Nucleoplasm
Cajal body Coilin RNA Splicing digestive oral and skin physiology Coiled Bodies Cell Biology snRNPs Biology Splicing Ribonucleoproteins Small Nuclear Cell biology TCERG1 Splicing factor Humans snRNP RNA Splicing Factors Histone locus body Transcriptional Elongation Factors Small nuclear ribonucleoprotein SnRNP Biogenesis |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1477-9137 |
| Popis: | Este artículo ha sido seleccionado para su inclusión en la sección “Research Highlights” de la revista. Cajal bodies are nuclear organelles involved in the nuclear phase ofsmall nuclear ribonucleoprotein (snRNP) biogenesis. In this study, weidentified the splicing factor TCERG1 as a coilin-associated factorthat is essential for Cajal body integrity. Knockdown of TCERG1disrupts the localization of the components of Cajal bodies, includingcoilin and NOLC1, with coilin being dispersed in the nucleoplasm intonumerous small foci, without affecting speckles, gems or the histonelocus body. Furthermore, the depletion of TCERG1 affects therecruitment of Sm proteins to uridine-rich small nuclear RNAs(snRNAs) to form the mature core snRNP. Taken together, theresults of this study suggest that TCERG1 plays an important role inCajal body formation and snRNP biogenesis. This work was supported by grants from the Spanish Ministry of Economy andCompetitiveness (Ministerio de Economı́a y Competitividad; grant numberBFU2017-89179-R to C.S. and grant number BFU2016-79699-P to C.H.M.) and theAndalusian Government (Excellence Project BIO-2515/2012) to C.S. Support fromthe European Region Development Fund [ERDF (FEDER)] is also acknowledged. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|