Application of fluorescence in situ hybridization to detect residual leukemic cells with 9;22 and 15;17 translocations

Autor: T. S. Kumaravel, Ryuzo Ohno, H Dohy, Mansyur Arif, Nanao Kamada, Ryuzo Ueda, T Kyo, Mariko Eguchi, Kimio Tanaka, K Iwato
Rok vydání: 1997
Předmět:
Zdroj: Leukemia. 11:436-440
ISSN: 1476-5551
0887-6924
Popis: We performed fluorescence in situ hybridization (FISH) upon 9;22 and 15;17 translocation-positive bone marrow cells to monitor the clinical course of 46 patients with chronic myelocytic leukemia (CML) and nine with acute promyelocytic leukemia (AML M3) who received chemotherapy and/or bone marrow transplantation (BMT). M-BCR-ABL and PML-RAR alpha probes were used to detect translocations of t(9;22) and t(15;17), respectively. Signals from CML patients treated with interferon (17 patients) or BMT (29 patients) were 0.5-15% positive for the 9;22 translocation. Among nine M3 patients who received extensive chemotherapy or BMT, 1-5% were positive for the 15;17 translocation. A highly sensitive FISH procedure using both translocation probes and a whole chromosome Y probe was established and applied to eight sex-mismatched BMT patients (seven CML and one AML M3), in which 0.1-0.6% of signals positive for the specific translocations were detected. These results suggested that interphase FISH is powerful enough to identify minor cell populations of 9;22 or 15;17 translocations after therapy, as well as to detect specific chromosome abnormalities at diagnosis.
Databáze: OpenAIRE