Application of fluorescence in situ hybridization to detect residual leukemic cells with 9;22 and 15;17 translocations
| Autor: | T. S. Kumaravel, Ryuzo Ohno, H Dohy, Mansyur Arif, Nanao Kamada, Ryuzo Ueda, T Kyo, Mariko Eguchi, Kimio Tanaka, K Iwato |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Acute promyelocytic leukemia
Cancer Research Pathology medicine.medical_specialty Neoplasm Residual Neutrophils Chromosomes Human Pair 22 Fusion Proteins bcr-abl Chromosomal translocation Biology Translocation Genetic Myelogenous Leukemia Promyelocytic Acute Bone Marrow Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases medicine Humans In Situ Hybridization Fluorescence Chromosomes Human Pair 15 Microscopy Confocal medicine.diagnostic_test Hybridization probe Hematology medicine.disease Minimal residual disease Leukemia medicine.anatomical_structure Oncology Cancer research Bone marrow Chromosomes Human Pair 9 DNA Probes Chromosomes Human Pair 17 Fluorescence in situ hybridization |
| Zdroj: | Leukemia. 11:436-440 |
| ISSN: | 1476-5551 0887-6924 |
| Popis: | We performed fluorescence in situ hybridization (FISH) upon 9;22 and 15;17 translocation-positive bone marrow cells to monitor the clinical course of 46 patients with chronic myelocytic leukemia (CML) and nine with acute promyelocytic leukemia (AML M3) who received chemotherapy and/or bone marrow transplantation (BMT). M-BCR-ABL and PML-RAR alpha probes were used to detect translocations of t(9;22) and t(15;17), respectively. Signals from CML patients treated with interferon (17 patients) or BMT (29 patients) were 0.5-15% positive for the 9;22 translocation. Among nine M3 patients who received extensive chemotherapy or BMT, 1-5% were positive for the 15;17 translocation. A highly sensitive FISH procedure using both translocation probes and a whole chromosome Y probe was established and applied to eight sex-mismatched BMT patients (seven CML and one AML M3), in which 0.1-0.6% of signals positive for the specific translocations were detected. These results suggested that interphase FISH is powerful enough to identify minor cell populations of 9;22 or 15;17 translocations after therapy, as well as to detect specific chromosome abnormalities at diagnosis. |
| Databáze: | OpenAIRE |
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