Thyroid hormone upregulates ecto-5′-nucleotidase/CD73 in C6 rat glioma cells
| Autor: | Emerson André Casali, Marcia Rosangela Wink, João José Freitas Sarkis, Elizandra Braganhol, Ana Maria Oliveira Battastini, Maria Luiza Morais Barreto-Chaves, Alessandra Sayuri Kikuchi Tamajusuku |
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| Rok vydání: | 2003 |
| Předmět: |
Purine
Biology Biochemistry chemistry.chemical_compound Endocrinology Cell Line Tumor Extracellular medicine Animals Secretion RNA Messenger 5'-Nucleotidase Molecular Biology chemistry.chemical_classification Regulation of gene expression Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Cell growth fungi Glioma Molecular biology Adenosine Rats Up-Regulation Thyroxine Enzyme chemistry Dactinomycin Triiodothyronine Hormone medicine.drug |
| Zdroj: | Molecular and Cellular Endocrinology. 205:107-114 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/s0303-7207(03)00197-7 |
| Popis: | Thyroid hormones have profound effects on the central nervous system, such as proliferation, secretion of growth factors and gene expression regulation. Ecto-NTPDases and ecto-5′-nucleotidase can control the extracellular ATP/adenosine levels, which have been described as proliferation factors. Here, we investigated the influence of T 3 on the enzyme cascade which catalyzes interconversion of purine nucleotides in rat C6 glioma cells. Exposure of C6 cells to T 3 caused a dose dependent increase of 30% in the AMP hydrolysis up to 0.25 nM, which was suppressed by actinomycin. No significant alteration was observed on ATP/ADP hydrolysis and T 4 at higher concentrations (10–1000 nM) promoted an increase in AMP hydrolysis that was not dose dependent. T 3 treatment also increased the expression of CD73 mRNA. Besides the importance of the ecto-5′-NT in the cell proliferation and differentiation, its overexpression can enhance extracellular adenosine levels, which could also be an important proliferation signal. |
| Databáze: | OpenAIRE |
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