Specific initiation of replication at the right-end telomere of the closed species of minute virus of mice replicative-form DNA
| Autor: | Jürg P. F. Nüesch, Andreas Q. Baldauf, Jean Rommelaere, Eleni Mumtsidu, Kurt Willwand |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA Replication
DNA clamp biology DNA polymerase viruses DNA polymerase II Immunology DNA replication Eukaryotic DNA replication Nicking enzyme Telomere biochemical phenomena metabolism and nutrition Virus Replication Microbiology Molecular biology Mice Virology Insect Science DNA Viral Minute Virus of Mice biology.protein Animals DNA supercoil Replication protein A Research Article |
| Zdroj: | Journal of Virology. 71:971-980 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.71.2.971-980.1997 |
| Popis: | We have developed an in vitro system that supports the replication of natural DNA templates of the autonomous parvovirus minute virus of mice (MVM). MVM virion DNA, a single-stranded molecule bracketed by short, terminal, self-complementary sequences, is converted into double-stranded replicative-form (RF) DNA when incubated in mouse A9 fibroblast extract. The 3' end of the newly synthesized complementary strand is ligated to the right-end hairpin of the virion strand, resulting in the formation of a covalently closed RF (cRF) molecule as the major conversion product. cRF DNA is not further replicated in A9 cell extract alone. On addition of purified MVM nonstructural protein NS1 expressed from recombinant baculoviruses or vaccinia viruses, cRF DNA is processed into a right-end (5' end of the virion strand) extended form (5'eRF). This is indicative of NS1-dependent nicking of the right-end hairpin at a distinct position, followed by unfolding of the hairpin and copying of the terminal sequence. In contrast, no resolution of the left-end hairpin can be detected in the presence of NS1. In the course of the right-end nicking reaction, NS1 gets covalently attached to the right-end telomere of the DNA product, as shown by immunoprecipitation with NS1-specific antibodies. The 5'eRF product is the target for additional rounds of NS1-induced nicking and displacement synthesis at the right end, arguing against the requirement of the hairpin structure for recognition of the DNA substrate by NS1. Further processing of the 5'eRF template in vitro leads to the formation of dimeric RF (dRF) DNA in a left-to-left-end configuration, presumably as a result of copying of the whole molecule by displacement synthesis initiated at the right-end telomere. Formation of dRF DNA is highly stimulated by NS1. The experimental results presented in this report support various assumptions of current models of parvovirus DNA replication and provide new insights into the replication functions of the NS1 protein. |
| Databáze: | OpenAIRE |
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