Ca2+enrichment in culture medium potentiates effect of oligonucleotides
| Autor: | Shunsuke Wada, Satoshi Obika, Reiko Waki, Fumito Wada, Shinichiro Hori, Tsuyoshi Yamamoto, Mio Noda |
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| Rok vydání: | 2015 |
| Předmět: |
Male
viruses Oligonucleotides Biology Transfection Cell Line Morpholinos In vivo Genetics Animals Humans Gene silencing Bridged nucleic acid RNA Small Interfering Cells Cultured Oligonucleotide Oligonucleotides Antisense Subcellular localization In vitro Culture Media Mice Inbred C57BL Biochemistry Cell culture Biophysics Nanoparticles Methods Online Calcium Plasmids |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkv626 |
| Popis: | Antisense and RNAi-related oligonucleotides have gained attention as laboratory tools and therapeutic agents based on their ability to manipulate biological events in vitro and in vivo. We show that Ca(2+) enrichment of medium (CEM) potentiates the in vitro activity of multiple types of oligonucleotides, independent of their net charge and modifications, in various cells. In addition, CEM reflects in vivo silencing activity more consistently than conventional transfection methods. Microscopic analysis reveals that CEM provides a subcellular localization pattern of oligonucleotides resembling that obtained by unassisted transfection, but with quantitative improvement. Highly monodispersed nanoparticles ~100 nm in size are found in Ca(2+)-enriched serum-containing medium regardless of the presence or absence of oligonucleotides. Transmission electron microscopy analysis reveals that the 100-nm particles are in fact an ensemble of much smaller nanoparticles (ϕ ∼ 15 nm). The presence of these nanoparticles is critical for the efficient uptake of various oligonucleotides. In contrast, CEM is ineffective for plasmids, which are readily transfected via the conventional calcium phosphate method. Collectively, CEM enables a more accurate prediction of the systemic activity of therapeutic oligonucleotides, while enhancing the broad usability of oligonucleotides in the laboratory. |
| Databáze: | OpenAIRE |
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