No evidence for a pathogen associated with pulmonary MALT lymphoma: a metagenomics investigation

Autor: Bruno Crestani, Valérie Caro, Marianne Kambouchner, Christophe Hennequin, Véronique Hourdel, Martine Antoine, Karen Leroy, Jacques Cadranel, Mathias Vandenbogaert, Hilario Nunes, Raphael Borie, Aurélie Cazes, Christiane Copie-Bergman, Aurélia Kwasiborski
Přispěvatelé: AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Environnement et Risques infectieux - Environment and Infectious Risks (ERI), Institut Pasteur [Paris], Hôpital Avicenne [AP-HP], Service de pneumologie [Avicenne], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'Anatomie et cytologie pathologiques [CHU Tenon], CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Physiopathologie et Epidémiologie des Maladies Respiratoires (PHERE (UMR_S_1152 / U1152)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Département de pathologie [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hopital Saint-Louis [AP-HP] (AP-HP), Centre de Recherche Saint-Antoine (CRSA), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Service de chirurgie thoracique [CHU Tenon], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), This work was supported by Assistance Publique- Hôpitaux de Paris (CRC 2013-006 DRCI APHP) and by Roche., Service d’Anatomie et cytologie pathologiques [CHU Tenon], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Centre de Recherche Saint-Antoine (CR Saint-Antoine), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Saint-Antoine [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Gestionnaire, HAL Sorbonne Université 5
Rok vydání: 2021
Předmět:
Cancer Research
Epidemiology
Short Report
lcsh:RC254-282
[SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
Virus
lcsh:Infectious and parasitic diseases
Microbiology
03 medical and health sciences
0302 clinical medicine
immune system diseases
hemic and lymphatic diseases
Medicine
lcsh:RC109-216
Pathogen
030304 developmental biology
0303 health sciences
Lung
biology
business.industry
RNA virus
MALT lymphoma
Achromobacter xylosoxidans
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
biology.organism_classification
medicine.disease
3. Good health
Lymphoma
Infectious Diseases
Lymphatic system
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
[SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract
business
Zdroj: Infectious Agents and Cancer
Infectious Agents and Cancer, BioMed Central, 2021, 16 (1), ⟨10.1186/s13027-021-00351-w⟩
Infectious Agents and Cancer, 2021, 16 (1), ⟨10.1186/s13027-021-00351-w⟩
Infectious Agents and Cancer, Vol 16, Iss 1, Pp 1-4 (2021)
ISSN: 1750-9378
Popis: Mucosa-associated lymphoid tissue (MALT) lymphoma is generally associated with chronic antigen stimulation: auto-antigens or of microbial origin. Only one study suggested association between Achromobacter xylosoxidans and pulmonary MALT lymphoma. We aimed to investigate the presence of virus or any infectious agents in pulmonary MALT lymphoma by using metagenomic next-generation sequencing (mNGS).All lung samples were centrally reviewed. The t(11;18) (q21;q21) was evaluated by FISH analysis. The snap frozen large lung biopsies were analyzed by mNGS. After lung biopsies homogenization total nucleic acids (RNA and DNA) were extracted, amplified and classified according to their taxonomic assignment, after exclusion of host DNA.We included 13 samples from pulmonary MALT lymphoma (mean age: 60.3 years, 7 women, 3 with auto-immune background) and 10 controls. The diagnosis of MALT lymphoma was confirmed for the 13 samples, 3 showed API2-MALT1 translocation (23%). No evidence of the presence of a specific pathogen was clearly identified in the group of patients with pulmonary MALT lymphoma. We identifiedA. xylosoxidans sequence in 4/13 patients and in 4/10 controls.This study did not find evidence for a DNA or RNA virus, a fungi, a parasite or a bacteria associated with pulmonary MALT lymphoma either in the stroma or in tumor cells.
Databáze: OpenAIRE
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