The ameliorative effect of bromelain on STZ-induced type 1 diabetes in rats through Oxi-LDL/LPA/LPAR1 pathway
Autor: | Salma M. Eraky, Nehal M. Ramadan, Nada F. Abo El-Magd |
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Rok vydání: | 2021 |
Předmět: |
Male
endocrine system diseases Bromelain (pharmacology) Serum albumin Pharmacology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Streptozocin Diabetes Mellitus Experimental chemistry.chemical_compound Diabetes mellitus Lysophosphatidic acid medicine Animals General Pharmacology Toxicology and Pharmaceutics Receptors Lysophosphatidic Acid biology Cholesterol business.industry General Medicine Malondialdehyde medicine.disease Streptozotocin Bromelains Rats Lipoproteins LDL Diabetes Mellitus Type 1 chemistry biology.protein Lysophospholipids business Oxidative stress Metabolic Networks and Pathways medicine.drug |
Zdroj: | Life sciences. 285 |
ISSN: | 1879-0631 |
Popis: | Aims Diabetes, a serious worldwide problem, is modulated via inflammation and oxidative stress. Bromelain, a natural compound, recently attracts interest due to its anti-inflammatory effects, while its mode of action remains not properly understood. Thus, investigating the antidiabetic effect of bromelain is promising. Materials and methods Rats were randomized into normal group, STZ group (were administrated single intraperitoneal (i.p) injection of 55 mg/kg streptozotocin (STZ)) and STZ + Bro group (were administrated single i.p injection of STZ, 72 h later were i.p administrated 10 mg/kg/day bromelain for 15 days). Wound healing ability was investigated for different groups. Spectrophotometry, ELISA, histopathological and immunohistochemical techniques were applied. Key findings Bromelain significantly decreased fasting blood glucose, serum triglycerides and cholesterol and hepatic malondialdehyde levels compared with STZ group. Moreover, Bromelain significantly increased serum albumin and total protein levels and percentage of wound healing compared with STZ group. These results were confirmed through the histopathological examination of liver, pancreas, and skin tissues. Investigating the molecular mechanism underlying these effects, STZ injection caused significant increase in hepatic oxidized-LDL (Oxi-LDL) and lysophosphatidic acid (LPA) levels and hepatic lysophosphatidic acid receptor 1 (LPAR1), and beta secretase (BACE1) protein tissue expressions, while bromelain significantly aborted these effects. Thus, STZ caused upregulation of Oxi-LDL/LPA/LPAR1/BACE1 pathway, while bromelain significantly ameliorated these effects. Significance To our best knowledge, this study represents the 1st study investigating Oxi-LDL/LPA/LPAR1/BACE1 pathway in STZ-induced diabetes in rats, in addition to the promising ameliorative effect of bromelain in STZ-induced diabetes in rats. |
Databáze: | OpenAIRE |
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