BDNF parabrachio-amygdaloid pathway in morphine-induced analgesia

Autor: Hugues Dardente, Pierre Veinante, Venetia Zachariou, Ralph J. DiLeone, Florent Barthas, Maysa Sarhan, Jennifer Kaufling, Sophie A. Pawlowski, Ipek Yalcin, Michel Barrot
Přispěvatelé: Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Pharamacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai [New York] (MSSM), Department of Psychiatry, Yale University [New Haven], Centre National de la Recherche Scientifique (UPR3212), University of Strasbourg, Institut des Neurosciences Cellulaires et Intégratives, Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2013
Předmět:
Male
Mice
extended amygdala
0302 clinical medicine
Neurotrophic factors
Pons
Neural Pathways
Medicine
pain
Pharmacology (medical)
Pain Measurement
0303 health sciences
biology
morphine
Dependovirus
Amygdala
Analgesics
Opioid
Psychiatry and Mental health
Nociception
medicine.anatomical_structure
Hyperalgesia
brain derived neurotrophic factor
Opiate
Proto-Oncogene Proteins c-fos
Neurotrophin
Pain Threshold
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
medicine.medical_specialty
Dark Adaptation
Mice
Transgenic
03 medical and health sciences
Extended amygdala
Internal medicine
Animals
Maze Learning
030304 developmental biology
Pharmacology
Brain-derived neurotrophic factor
Parabrachial Nucleus
business.industry
Brain-Derived Neurotrophic Factor
Mice
Inbred C57BL
parabrachio amygdaloid
Endocrinology
nervous system
Rotarod Performance Test
Exploratory Behavior
biology.protein
business
Neuroscience
030217 neurology & neurosurgery
Zdroj: International Journal of Neuropsychopharmacology
International Journal of Neuropsychopharmacology, Oxford University Press (OUP), 2013, 16 (7), pp.1649-1660. ⟨10.1017/S146114571200168X⟩
ISSN: 1469-5111
1461-1457
DOI: 10.1017/s146114571200168x
Popis: In addition to its neurotrophic role, brain-derived neurotrophic factor (BDNF) is involved in a wide array of functions, including anxiety and pain. The central amygdaloid nucleus (CeA) contains a high concentration of BDNF in terminals, originating from the pontine parabrachial nucleus. Since the spino-parabrachio-amygdaloid neural pathway is known to convey nociceptive information, we hypothesized a possible involvement of BDNF in supraspinal pain-related processes. To test this hypothesis, we generated localized deletion of BDNF in the parabrachial nucleus using local bilateral injections of adeno-associated viruses in adult floxed-BDNF mice. Basal thresholds of thermal and mechanical nociceptive responses were not altered by BDNF loss and no behavioural deficit was noticed in anxiety and motor tests. However, BDNF-deleted animals displayed a major decrease in the analgesic effect of morphine. In addition, intra-CeA injections of the BDNF scavenger TrkB-Fc in control mice also decreased morphine-induced analgesia. Finally, the number of c-Fos immunoreactive nuclei after acute morphine injection was decreased by 45% in the extended amygdala of BDNF-deleted animals. The absence of BDNF in the parabrachial nucleus thus altered the parabrachio-amygdaloid pathway. Overall, our study provides evidence that BDNF produced in the parabrachial nucleus modulates the functions of the parabrachio-amygdaloid pathway in opiate analgesia.
Databáze: OpenAIRE
Externí odkaz: