Bombesin conjugated solid lipid nanoparticles for improved delivery of epigallocatechin gallate for breast cancer treatment
| Autor: | Halley Gora Ravuri, Deep Pooja, Hitesh Kulhari, Sagarika Gudem, Suresh K. Bhargava, Rasika Radhakrishnan, Sistla Ramakrishna |
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| Rok vydání: | 2019 |
| Předmět: |
Cell Survival
Surface Properties Antineoplastic Agents Breast Neoplasms Epigallocatechin gallate Biochemistry Catechin chemistry.chemical_compound Nanocapsules Cell Line Tumor Gastrin-releasing peptide Lecithins Solid lipid nanoparticle Animals Humans Cytotoxicity Molecular Biology Chemistry Organic Chemistry Bombesin Biological Transport Neoplasms Experimental Cell Biology Mice Inbred C57BL Drug Liberation Targeted drug delivery Delayed-Action Preparations Liposomes Drug delivery Cancer research Female sense organs Drug carrier Stearic Acids |
| Zdroj: | Chemistry and Physics of Lipids. 224:104770 |
| ISSN: | 0009-3084 |
| Popis: | Epigallocatechin-gallate (EGCG) is a potent anti-cancer therapeutic which effectively controls the growth of cancerous cells through a variety of different pathways. However, its molecular structure is susceptible to modifications due to cellular enzymes affecting its stability, bioavailability and hence, overall efficiency. In this study, we have initially encapsulated EGCG in the matrix of solid lipid nanoparticles to provide a stable drug carrier. To confer additional specificity towards gastrin releasing peptide receptors (GRPR) overexpressed in breast cancer, EGCG loaded nanoparticles were conjugated with a GRPR-specific peptide. In-vitro cytotoxicity studies showed that the peptide-conjugated formulations possessed greater cytotoxicity to cancer cell lines compared to the non-conjugated formulations. Further, in-vivo studies performed on C57/BL6 mice showed greater survivability and reduction in tumour volume in mice treated with peptide-conjugated formulation as compared to the mice treated with non-conjugated formulation or with plain EGCG. These results warrant the potential of the system designed in this study as a novel and effective drug delivery system in breast cancer therapy. |
| Databáze: | OpenAIRE |
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