Hepatocyte growth factor induces pErk and pSTAT3 (Ser 727) to promote mitochondrial activity and neurite outgrowth in primary dorsal root ganglion cultures
| Autor: | Hana Seung, Sang Ho Kwon, Junghun Lee, Jaegook Lim, Mi Young Lee, Sun-Young Kim, Nayeon Lee |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway STAT3 Transcription Factor Neurite Sensory Receptor Cells Neuronal Outgrowth Primary Cell Culture Mitochondrion Serine 03 medical and health sciences Mice 0302 clinical medicine Dorsal root ganglion Peripheral Nerve Injuries Ganglia Spinal Nitriles medicine Butadienes Animals Enzyme Inhibitors Phosphorylation STAT3 Extracellular Signal-Regulated MAP Kinases Electron Transport Complex I biology Chemistry Hepatocyte Growth Factor General Neuroscience Regeneration (biology) Receptor Protein-Tyrosine Kinases Axons Cell biology Mitochondria Nerve Regeneration Protein Transport 030104 developmental biology medicine.anatomical_structure biology.protein Hepatocyte growth factor 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neuroreport. 32(6) |
| ISSN: | 1473-558X |
| Popis: | Hepatocyte growth factor (HGF) promotes the neurite outgrowth of sensory neurons in developmental stages, but its role in injured peripheral nerves in adult mice remains largely been unexplored. In this study, we investigated the role of HGF in the regeneration of injured peripheral nerves using cultured dorsal root ganglions (DRGs). When cells were treated with HGF protein, the length of the neurite was increased 1.4-fold compared to the untreated control group. HGF greatly increased the level of phosphorylated STAT3 at serine 727 [pSTAT3 (Ser 727)], thereby translocating the protein to the mitochondria. HGF treatment increased the activity of mitochondrial complex I. When DRGs were cultured in the presence of U0126, a pharmacological inhibitor of Erk, the HGF-mediated increase in neurite outgrowth and the level of pSTAT3 (Ser 727) were both suppressed. Taken together, these results suggest that the HGF/c-met pathway might promote neurite outgrowth by controlling mitochondrial activity through the HGF/Erk/STAT3 axis. |
| Databáze: | OpenAIRE |
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