Leaf heteroblasty in Passiflora edulis as revealed by metabolic profiling and expression analyses of the microRNAs miR156 and miR172
| Autor: | Diego Silva Batista, Wagner Campos Otoni, João Henrique F. Cavalcanti, Lorena Melo Vieira, Priscila Oliveira Silva, Amanda Mendes Fernandes, Dimas M. Ribeiro, Andréa Dias Koehler, Carlos Hernán Barrera-Rojas, Fabio Tebaldi Silveira Nogueira |
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| Přispěvatelé: | Universidade Federal de Viçosa (UFV), Universidade Estadual Do Maranhão, Universidade Federal Do Amazonas, Universidade de São Paulo (USP), Universidade Estadual Paulista (UNESP) |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Perennial plant Arabidopsis Organogenesis Plant Science 01 natural sciences Passiflora 03 medical and health sciences Gene Expression Regulation Plant Botany Metabolome De novo shoot organogenesis Juvenile Sugar biology fungi food and beverages EXPRESSÃO GÊNICA MicroRNA Original Articles Meristem Heteroblasty biology.organism_classification Passiflora edulis Plant Leaves MicroRNAs 030104 developmental biology Shoot 010606 plant biology & botany |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV Ann Bot Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-28T19:27:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-07-08 Background and Aims: Juvenile-to-adult phase transition is marked by changes in leaf morphology, mostly due to the temporal development of the shoot apical meristem, a phenomenon known as heteroblasty. Sugars and microRNA-controlled modules are components of the heteroblastic process in Arabidopsis thaliana leaves. However, our understanding about their roles during phase-changing in other species, such as Passiflora edulis, remains limited. Unlike Arabidopsis, P. edulis (a semi-woody perennial climbing vine) undergoes remarkable changes in leaf morphology throughout juvenile-to-adult transition. Nonetheless, the underlying molecular mechanisms are unknown. Methods: Here we evaluated the molecular mechanisms underlying the heteroblastic process by analysing the temporal expression of microRNAs and targets in leaves as well as the leaf metabolome during P. edulis development. Key Results: Metabolic profiling revealed a unique composition of metabolites associated with leaf heteroblasty. Increasing levels of glucose and α-trehalose were observed during juvenile-to-adult phase transition. Accumulation of microRNA156 (miR156) correlated with juvenile leaf traits, whilst miR172 transcript accumulation was associated with leaf adult traits. Importantly, glucose may mediate adult leaf characteristics during de novo shoot organogenesis by modulating miR156-targeted PeSPL9 expression levels at early stages of shoot development. Conclusions: Altogether, our results suggest that specific sugars may act as co-regulators, along with two microRNAs, leading to leaf morphological modifications throughout juvenile-to-adult phase transition in P. edulis. Departamento de Biologia Vegetal/Instituto de Biotecnologia Aplicada A Agropecuária (BIOAGRO) Universidade Federal de Viçosa Universidade Estadual Do Maranhão Departamento de Biologia Vegetal Universidade Federal de Viçosa Instituto de Educação Agricultura e Ambiente Universidade Federal Do Amazonas Escola Superior de Agricultura Luiz de Queiroz Universidade de São Paulo Instituto de Biociências Universidade Estadual de São Paulo Instituto de Biociências Universidade Estadual de São Paulo |
| Databáze: | OpenAIRE |
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